Effects of 3-benzidino-6-phenylpyridazine, as an acetylcholinesterase inhibitor, on outward potassium current in acutely isolated rat hippocampal pyramidal neurons
| Autor: | Miao-Yu Li, Huizhi Du, Pin Yang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Patch-Clamp Techniques
Potassium Channels medicine.drug_class Potassium Hippocampus chemistry.chemical_element Hippocampal formation Toxicology chemistry.chemical_compound medicine Animals Patch clamp Rats Wistar biology Dose-Response Relationship Drug Pyramidal Cells General Medicine biology.organism_classification Acetylcholinesterase Electric eel Rats Pyridazines Electrophysiology Biochemistry Acetylcholinesterase inhibitor chemistry Electrophorus Biophysics Cholinesterase Inhibitors Delayed Rectifier Potassium Channels |
| Zdroj: | Toxicology letters. 181(2) |
| ISSN: | 0378-4274 |
| Popis: | As an acetylcholinesterase (AChE) inhibitor, the effects of 3-benzidino-6-phenylpyridazine (BPP) on outward potassium current including delayed rectifier potassium current (I(K(DR))) and transient outward potassium current (I(K(A))) in acutely isolated rat hippocampal pyramidal neurons were studied, using the whole cell patch-clamp technique. BPP reversibly inhibited electric eel AChE as an inhibitor, with IC(50) of 1.43 microM. BPP (0.10-100 microM) decreased I(K(DR)) and I(K(A)) in a concentration-dependent, voltage-independent and partial reversible manner, with IC(50) of 0.47 and 0.31 microM, respectively. 10 microM BPP did not affect steady-state activation of I(K(DR)) and I(K(A)). In addition, 10 microM BPP shifted the voltage dependence of steady-state inactivation of I(K(A)) towards negative potential. In conclusion, BPP potently inhibits I(K(DR)) and I(K(A)) in rat hippocampal pyramidal neurons, which may contribute to BPP's restoring the damaged central nervous system. |
| Databáze: | OpenAIRE |
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