Development of an automated on-line pepsin digestion–liquid chromatography–tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents
| Autor: | Albert G. Hulst, Jeroen Carol-Visser, Ben L. M. van Baar, A. Fidder, Daan Noort, Marcel J. van der Schans, Hubertus Irth |
|---|---|
| Přispěvatelé: | Organic Chemistry, BioAnalytical Chemistry |
| Rok vydání: | 2008 |
| Předmět: |
Chemical Warfare Agents
Sarin Protein adducts Clinical Biochemistry Mass spectrometry Online Systems Biochemistry Analytical Chemistry Adduct chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Mustard Gas medicine Cholinesterases Humans Serum Albumin Chromatography Chemistry Sulfur mustard Cell Biology General Medicine Human serum albumin Pepsin A Chromatography Liquid medicine.drug |
| Zdroj: | Carol-Visser, J, van der Schans, M, Fidder, A, Huist, A G, van Baar, B L M, Irth, H & Noort, D 2008, ' Development of an automated pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents ', Journal of Chromatography B, vol. 870, no. 1, pp. 91-97 . https://doi.org/10.1016/j.jchromb.2008.06.008 Journal of Chromatography B, 870(1), 91-97. Elsevier |
| ISSN: | 1570-0232 |
| Popis: | Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively. © 2008 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect