Cell proliferation patterns andp53 expression in gastric dysplasia
| Autor: | Donatella Spina, Piero Tosi, Carla Vindigni, Clelia Miracco, M. I. Filipe |
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| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Middle layer education Stomach Diseases Adenocarcinoma Stomach Neoplasms Proliferating Cell Nuclear Antigen Nucleolus Organizer Region medicine Humans P53 expression biology Cell growth Stomach Antibodies Monoclonal Discriminant Analysis Nuclear Proteins Genes p53 medicine.disease digestive system diseases Neoplasm Proteins Proliferating cell nuclear antigen Gastric Dysplasia Ki-67 Antigen surgical procedures operative medicine.anatomical_structure Oncology Gastric Mucosa Dysplasia biology.protein Nucleolus organizer region Precancerous Conditions Cell Division |
| Zdroj: | International Journal of Cancer. 62:149-154 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910620207 |
| Popis: | Gastric dysplasia (high-grade, HGD, and low-grade, LGD) and normal mucosa were tested for anti-p53, anti-Ki-67 and anti-PCNA monoclonal antibodies on paraffin sections, and for relative AgNOR area and number on semithin Epon-Araldite sections. The proliferative compartment in normal mucosa was restricted to the middle layer corresponding to the neck-isthmus region. In LGD and HGD there was an expansion of this compartment to the lower and upper layers of mucosa, and in HGD in particular to the upper layer. p53 was always negative in LGD as well as in normal mucosa, while it was positive in 34 out of 51 cases of HGD. The most discriminant variables between LGD and HGD were relative AgNOR area and the percentages of MIB-1, p53 and PCNA. In p53-positive HGD the highest percentages of PCNA and MIB-1 were in the middle and upper layers (PCNA) or the upper layer (MIB-1), while in p53-negative HGD cases cell proliferation was maximal in the middle layer, although also present in the upper layer. The majority of cases of LGD did not demonstrate cell proliferation in the upper layer, but 5 cases behaved similarly to the p53-negative HGD cases. No significant correlations were found among percentages of MIB-1 and of PCNA and relative AgNOR area and number. |
| Databáze: | OpenAIRE |
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