Fate of D3 mouse embryonic stem cells exposed to X-rays or carbon ions

Autor: Elena Nasonova, S. Luft, Onetsine Arrizabalaga, Sylvia Ritter, Marco Durante, Alexander Helm, Diana Pignalosa
Přispěvatelé: Luft, S., Pignalosa, Diana, Nasonova, E., Arrizabalaga, O., Helm, A., Durante, Marco, Ritter, S.
Rok vydání: 2013
Předmět:
Time Factors
Health
Toxicology and Mutagenesis
Chromosomal translocation
Ionizing radiation
Mice
Heavy Ions
Myocytes
Cardiac
Heavy Ion
In Situ Hybridization
Fluorescence
Genetics
Medicine (all)
G2 Phase Cell Cycle Checkpoint
Cell Differentiation
Clonogenic cell
Flow Cytometry
Carbon ion
Cell biology
G2 Phase Cell Cycle Checkpoints
Cardiomyocyte formation
Pluripotency
Pluripotent Stem Cells
Time Factor
Cell Survival
Blotting
Western
chemistry.chemical_element
SOXB1 Transcription Factor
Biology
Chromosome Aberration
Cell Line
Genetic
Embryonic Stem Cell
Animals
Irradiation
Embryonic Stem Cells
Chromosome Aberrations
Pluripotent Stem Cell
Animal
SOXB1 Transcription Factors
X-Rays
Embryogenesis
Chromosome
Dose-Response Relationship
Radiation
Embryonic stem cell
Carbon
Health
Toxicology and Mutagenesi
chemistry
X-Ray
Octamer Transcription Factor-3
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis. 760
ISSN: 1383-5718
Popis: The risk of radiation exposure during embryonic development is still a major problem in radiotoxicology. In this study we investigated the response of the murine embryonic stem cell (mESC) line D3 to two radiation qualities: sparsely ionizing X-rays and densely ionizing carbon ions. We analyzed clonogenic cell survival, proliferation, induction of chromosome aberrations as well as the capability of cells to differentiate to beating cardiomyocytes up to 3 days after exposure. Our results show that, for all endpoints investigated, carbon ions are more effective than X-rays at the same radiation dose. Additionally, in long term studies (≥8 days post-irradiation) chromosomal damage and the pluripotency state were investigated. These studies reveal that pluripotency markers are present in the progeny of cells surviving the exposure to both radiation types. However, only in the progeny of X-ray exposed cells the aberration frequency was comparable to that of the control population, while the progeny of carbon ion irradiated cells harbored significantly more aberrations than the control, generally translocations. We conclude that cells surviving the radiation exposure maintain pluripotency but may carry stable chromosomal rearrangements after densely ionizing radiation.
Databáze: OpenAIRE
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