DNA-binding, cytotoxicity, cellular uptake, apoptosis and photocleavage studies of Ru(II) complexes
| Autor: | D. Anil Kumar, Y. Praveen Kumar, P. Venkat Reddy, Sirasani Satyanarayana, Surya S. Singh, N. Deepika, C. Shobha Devi, K. Laxma Reddy |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Base pair
Stereochemistry Phenanthroline Phenazine Biophysics Apoptosis 010402 general chemistry 01 natural sciences HeLa chemistry.chemical_compound Anti-Infective Agents DU145 Cell Line Tumor Humans Radiology Nuclear Medicine and imaging Cytotoxicity Radiation Radiological and Ultrasound Technology biology Viscosity 010405 organic chemistry Chemistry DNA Flow Cytometry Photochemical Processes biology.organism_classification 0104 chemical sciences Ruthenium Compounds Spectrophotometry Ultraviolet |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 160:142-153 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2016.03.061 |
| Popis: | Two Ru(II) complexes [Ru(phen)2bppp](ClO4)2 (1) and [Ru(phen)27-Br-dppz](ClO4)2 (2) [phen=1,10 phenanthroline, 7-Br-dppz=7-fluorodipyrido[3,2-a:2',3'-c]phenazine, bppp=11-bromo-pyrido[2',3':5,6]pyrazino[2,3-f] [1,10]phenanthroline] have been synthesized and characterized by elemental analysis, ES-MS, (1)H-NMR, (13)C-NMR and IR. The in vitro cytotoxicity of the complexes examined against a panel of cancer cell lines (HeLa, Du145 and A549) by MTT method, both complexes show prominent anticancer activity against various cancer cells. Live cell imaging study and flow cytometric analysis demonstrate that both the complexes 1 and 2 could cross the cell membrane accumulating in the nucleus. Further, flow cytometry experiments showed that the cytotoxic Ru(II) complexes 1 and 2 induced apoptosis of HeLa tumor cell lines. Photo induced DNA cleavage studies have been performed and results indicate that both the complexes efficiently photo cleave pBR322 DNA. The binding properties of two complexes toward CT-DNA were investigated by various optical methods and viscosity measurements. The experimental results suggested that both Ru(II) complexes can intercalate into DNA base pairs. The complexes were docked into DNA-base pairs using the GOLD docking program. |
| Databáze: | OpenAIRE |
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