Poricoic acid A induces apoptosis and autophagy in ovarian cancer via modulating the mTOR/p70s6k signaling axis
| Autor: | Jin Xu, Ma Rui, Xueqi Liang, Qiang Zhao, Zhang Zhenhua |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
Physiology Poria cocos QH301-705.5 Immunology Biophysics Mice Nude Ocean Engineering Apoptosis medicine.disease_cause Biochemistry Flow cytometry Mice R5-920 Ovarian cancer Cell Line Tumor medicine Autophagy Animals Humans Viability assay General Pharmacology Toxicology and Pharmaceutics Biology (General) PI3K/AKT/mTOR pathway Cell Proliferation Ovarian Neoplasms medicine.diagnostic_test PAA Chemistry General Neuroscience TOR Serine-Threonine Kinases Ribosomal Protein S6 Kinases 70-kDa Cell migration Cell Biology General Medicine biochemical phenomena metabolism and nutrition medicine.disease Triterpenes mTOR signaling pathway Cancer research Female MHY1485 Carcinogenesis Research Article |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 54, Iss 12 (2021) Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Volume: 54, Issue: 12, Article number: e11183, Published: 18 OCT 2021 Brazilian Journal of Medical and Biological Research v.54 n.12 2021 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | Due to the high mortality and rapid disease progression, ovarian cancer remains one of the most common malignancies threatening the health of women. The present study was conducted to explore the anticancer effects and the underlying mechanisms of poricoic acid A (PAA), the main components of Poria cocos, on ovarian cancer. We investigated the anticancer effects of different concentrations of PAA in the SKOV3 cell line. Cell viability and proliferation were examined by CCK-8 assay. Cellular migration and invasion were assessed by the scratch and Transwell migration assays, respectively. The effect of PPA on cell apoptosis was measured by flow cytometry and caspase-3/8/9 colorimetric assay. Western blot was performed to detect protein level changes related to apoptosis and mTOR signaling pathways. The in vivo anticancer effect of PAA was evaluated using xenograft tumorigenesis model in nude mice. Our results showed that PAA suppressed SKOV3 cellular viability, migration, and invasion in a dosage-dependent manner. Flow cytometry results demonstrated PAA treatment could induce SKOV3 cell apoptosis. In addition, increased ratio of LC3-II/LC3-I (a marker for autophagosome formation) was observed after PAA treatment, as well as inhibition of m-TOR and p70s6k phosphorylation. In nude mice, PAA treatment reduced the xenograft tumor weight by 70% (P |
| Databáze: | OpenAIRE |
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