Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara

Autor: Philippe de Lajudie, Khaled Chaich, Souhir Soussou, Noureddine Bouras, Lucette Maure, Abdelkader Bekki, Michael D. Holtz, Brigitte Brunel, Jean Claude Cleyet-Marel
Přispěvatelé: Laboratoire Génie de l’eau et de l’Environnement en Milieu Saharien, Université de Ouargla, Laboratoire de Microbiologie, Faculté des Sciences, University of Oran Es-Sénia [Oran], École normale supérieure - Kouba-Alger (ENS Kouba-Alger), Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, Alberta Agriculture and Rural Development, Field Crop Development Centre (FCDC), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Fertil'Innov Environnement, University of Oran Es-Senia [Oran] | Université d'Oran Es-Senia [Oran], Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
phylogénétique
sol
ved/biology.organism_classification_rank.species
algérie
Shrub
stabilisation du sol
Spontaneous legume
caractérisation génétique
N-2-fixation
genista saharae
biology
soil stabilization
Mesorhizobium
legume
genêtic variation
spontaneous legume
fixation de l'azote
Sinorhizobium
diversité génétique
General Agricultural and Biological Sciences
Algerian Sahara
food.ingredient
030106 microbiology
Drought tolerance
housekeeping genes
Genista saharae
légumineuse
soil
dune
03 medical and health sciences
food
Botany
Genetic variation
Genetic diversity
Housekeeping genes
desertization
sahara
préservation de l'environnement
ved/biology
rhizobial diversity
15. Life on land
biology.organism_classification
16S ribosomal RNA
030104 developmental biology
Neorhizobium
Rhizobial diversity
désertification
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Zdroj: Symbiosis
Symbiosis, Springer Verlag, 2017, 71 (2), pp.111-120. ⟨10.1007/s13199-016-0414-y⟩
ISSN: 0334-5114
DOI: 10.1007/s13199-016-0414-y⟩
Popis: Genista saharae is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N-2-fixation. In this study, the root nodule bacteria of G. saharae were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate G. saharae species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (atpD, recA and 16S rRNA). The majority of isolates (81 %) belonged to the genus Ensifer (previously Sinorhizobium), represented mainly by the species Ensifer meliloti. The next most abundant genera were Neorhizobium (17 %) with 3 different species: N. alkalisoli, N. galegae and N. huautlense and Mesorhizobium (1.75 %) represented by the species M. camelthorni. Most of the isolated strains tolerated up to 4%(w/v) NaCl and grew at 45 degrees C. This study is the first report on the characterization of G. saharae microsymbionts in the Algerian Sahara.
Databáze: OpenAIRE
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