Production of Recombinant Bleaching Enzymes from Thermophilic Microorganisms in Fungal Hosts
| Autor: | Fabrícia Paula de Faria, Maristela Azevedo, V. S. Junior Te'o, K. M. Helena Nevalainen, Angela Cziferszky, Peter L. Bergquist, Moreland D. Gibbs |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular Hot Temperature Protein Conformation Molecular Sequence Data Bioengineering Protein Sorting Signals Polymerase Chain Reaction Applied Microbiology and Biotechnology Biochemistry Microbiology Kluyveromyces Plasmid Amino Acid Sequence Molecular Biology Trichoderma reesei Trichoderma Kluyveromyces lactis Expression vector Bacteria biology Thermophile Fungi General Medicine biology.organism_classification Recombinant Proteins Yeast Enzymes Xylan Endo-1 3-beta-Xylosidase Xylosidases Heterologous expression Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. :165-176 |
| ISSN: | 0273-2289 |
| DOI: | 10.1385/abab:98-100:1-9:165 |
| Popis: | Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2mu-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 x HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins. |
| Databáze: | OpenAIRE |
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