Infrequent somatic deletion of the 5' region of the COL1A2 gene in oesophageal squamous cell cancer patients
| Autor: | M. Iqbal Parker, E. Dietzsch |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Esophageal Neoplasms Clinical Biochemistry Loss of Heterozygosity Biology medicine.disease_cause Polymerase Chain Reaction Collagen Type I law.invention Loss of heterozygosity law medicine Humans Allele Dinucleotide Repeats Promoter Regions Genetic Gene Polymerase chain reaction Aged Polymorphism Genetic Biochemistry (medical) General Medicine Middle Aged Molecular biology Introns Procollagen peptidase Epidermoid carcinoma Carcinoma Squamous Cell Microsatellite Autoradiography Female Collagen Carcinogenesis 5' Untranslated Regions Gene Deletion Microsatellite Repeats |
| Zdroj: | Clinical chemistry and laboratory medicine. 40(9) |
| ISSN: | 1434-6621 |
| Popis: | Oesophageal squamous cell cancer is the leading cause of cancer death amongst African males in South Africa. DNA was isolated from normal and tumour biopsies of the oesophagi of 33 African patients with squamous cell carcinoma of the oesophagus and was analysed with two dinucleotide repeat polymorphisms, a GT repeat sequence in the first intron, and a CA repeat in the promoter of the human alpha2(I) procollagen gene (COL1A2), using the polymerase chain reaction (PCR). Normal and tumour DNAs from each individual were compared to identify changes present in the tumour DNA, but absent in normal DNA. Twenty two cases were informative (heterozygous) for the promoter polymorphism and 24 cases were informative for the intronic polymorphism. Loss of heterozygosity (LOH) was seen in 2/22 (9.1%) for the promoter and 3/24 (12.5%) for the intronic polymorphism. These changes involved a total of three patients: two patients displayed the lost allele incorporating both the CA repeat and GT repeat loci; the third patient revealed LOH at the intronic polymorphism, but was non-informative (homozygous) for the promoter polymorphism. Deletions within the procollagen genes may represent an as yet unrecognised but rare event in the multistep process of carcinogenesis. |
| Databáze: | OpenAIRE |
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