Efficient one-step direct labelling of recombinant antibodies with technetium-99m
| Autor: | Giovanni Neri, Fabio Ponzo, Anna Paola Iurilli, Mauro Liberatore, Dario Neri, Alessandro Pini, Antonio Centi Colella, Alessandro Pala, Fabrizio Padula, Giuseppe Spampinato |
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| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Immunoconjugates biology Antibody Affinity Technetium Peptide General Medicine Antibodies Bacterial Recombinant Proteins In vitro Residue (chemistry) Recombinant antibodies chemistry Biochemistry Isotope Labeling Labelling biology.protein Radiology Nuclear Medicine and imaging Antibody Immunoglobulin Fragments Technetium-99m direct labelling method recombinant antibodies technetium-99m labelling Cysteine |
| Zdroj: | European Journal of Nuclear Medicine. 22:1326-1329 |
| ISSN: | 1619-7089 0340-6997 |
| DOI: | 10.1007/bf00801622 |
| Popis: | High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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