Pros and cons of direct genotyping on tuberculosis clinical samples
| Autor: | Hamidreza Sadegh, Farid Abdolrahimi, Ghazaleh Farmanfarmaei, Seyed Davar Siadat, Farzam Vaziri, Abolfazl Fateh, Morteza Masoumi, Nayereh Ebrahimzadeh, Fatemeh Rahimi Jamnani, Mansour Kargarpour Kamakoli |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Partially successful medicine.medical_specialty Tuberculosis Genotype Genotyping Techniques 030106 microbiology Minisatellite Repeats Biology Microbiology Mycobacterium tuberculosis 03 medical and health sciences Molecular genetics Epidemiology medicine Humans Genotyping Alleles Retrospective Studies Molecular Epidemiology cons Genetic Variation medicine.disease biology.organism_classification Virology Bacterial Typing Techniques Infectious Diseases Mixed infection |
| Zdroj: | Microbial Pathogenesis. 103:135-138 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2016.12.025 |
| Popis: | Objective Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). Methods We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients. Results There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load (P = 0.001). VNTR loci were amplified successfully for 41.5% of the clinical samples (19–24 loci), 32.6% (13–18 loci), 23.7% (7–12 loci) and 2.2% (1–6 loci). Loci of 2401, 577, 2996 and 154 had the highest power to show the mixed strains infection in clinical samples. Conclusions Direct MIRU-VNTR is partially successful in complete genotyping of M. tuberculosis strains. On the other hand, detection of polyclonal infection is undoubtedly reliable based on the direct MIRU-VNTR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect