Tracing goes viral: Viruses that introduce expression of fluorescent proteins in chemically-specific neurons
| Autor: | Sybren F. de Kloet, Antonio Luchicchi, Tommy Pattij, John Noel M. Viaña, Nathan J. Marchant |
|---|---|
| Přispěvatelé: | Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience - Brain Imaging, Integrative Neurophysiology, Amsterdam Neuroscience - Compulsivity, Impulsivity & Attention, Anatomy and neurosciences, Amsterdam Neuroscience - Systems & Network Neuroscience |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Neurons Computer science General Neuroscience Genetic Vectors Neurotransmitter systems Research needs Chemogenetics Optogenetics Tracing Dependovirus Retrograde tracing Viral vector 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Dogs Expression (architecture) Viruses Animals Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Methods, 348:109004. Elsevier Luchicchi, A, Pattij, T, Viaña, J N M, de Kloet, S & Marchant, N 2021, ' Tracing goes viral : Viruses that introduce expression of fluorescent proteins in chemically-specific neurons ', Journal of Neuroscience Methods, vol. 348, 109004 . https://doi.org/10.1016/j.jneumeth.2020.109004 Luchicchi, A, Pattij, T, Viaña, J N M, de Kloet, S & Marchant, N 2021, ' Tracing goes viral: Viruses that introduce expression of fluorescent proteins in chemically-specific neurons ', Journal of Neuroscience Methods, vol. 348, 109004 . https://doi.org/10.1016/j.jneumeth.2020.109004 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2020.109004 |
| Popis: | © 2020Over the last century, there has been great progress in understanding how the brain works. In particular, the last two decades have been crucial in gaining more awareness over the complex functioning of neurotransmitter systems. The use of viral vectors in neuroscience has been pivotal for such development. Exploiting the properties of viral particles, modifying them according to the research needs, and making them target chemically-specific neurons, techniques such as optogenetics and chemogenetics have been developed, which could lead to a giant step toward gene therapy for brain disorders. In this review, we aim to provide an overview of some of the most widely used viral techniques in neuroscience. We will discuss advantages and disadvantages of these methods. In particular, attention is dedicated to the pivotal role played by the introduction of adeno-associated virus and the retrograde tracer canine-associated-2 Cre virus in order to achieve optimal visualization, and interrogation, of chemically-specific neuronal populations and their projections. |
| Databáze: | OpenAIRE |
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