Inhibition of α4 integrin mediated adhesion was involved in the reduction of B16-F10 melanoma cells lung colonization in C57BL/6 mice treated with Gambogic acid
| Autor: | Yong Yang, Wei Wang, Na Lu, Qi Qi, Hong-Yan Gu, Lei Qiang, Wei Liu, Qi-Dong You, Qinglong Guo, Jie Zhao, Ling-Bo Zhang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Neoplasms Cell Survival Integrin alpha4 Xanthones Blotting Western Cell Integrin Melanoma Experimental Down-Regulation Biology Inhibitory Concentration 50 Mice chemistry.chemical_compound Cell Movement In vivo Cell Line Tumor Cell Adhesion medicine Animals Pharmacology Dose-Response Relationship Drug Cell adhesion molecule Flow Cytometry Antineoplastic Agents Phytogenic In vitro Mice Inbred C57BL Blot medicine.anatomical_structure chemistry Cell culture Cancer research biology.protein Gambogic acid |
| Zdroj: | European Journal of Pharmacology. 589:127-131 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2008.04.063 |
| Popis: | Gambogic acid, the major active ingredient of gamboge, has been shown to exhibit anti-cancer activity both in vivo and in vitro. However, its potential activity in tumor metastasis remains unclear. In the present study, we found that gambogic acid strongly inhibited the adhesion of highly metastatic mouse melanoma B16-F10 cells in vitro. Gambogic acid also exhibited significant anti-metastasis activity on the development of in vivo artificial metastases (i.e. following tail vein i.v. injection of the B16-F10 melanoma tumor cells in C57BL/6 mice). Flow cytometric analysis and Western blot showed that gambogic acid inhibited the cell surface expression of alpha(4) integrin, while exhibited negligible effects on the expression of alpha(5) and beta(1) integrin. Thus we concluded for the first time that gambogic acid could inhibit the adhesion and migration of B16-F10 cells in vitro and in vivo, in which down-regulation of alpha(4) integrin expression was involved. |
| Databáze: | OpenAIRE |
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