American cockroach Cr-PI allergen induces lymphocyte proliferation and cytokine production in atopic patients
| Autor: | K. C. G. Jeng, C. H. Wu, D. W. Wong, J. L. Lan, M. T. Liu |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Allergy biology medicine.medical_treatment Immunology Lymphocyte proliferation medicine.disease_cause Immunoglobulin E medicine.disease Atopy Allergen Endocrinology Cytokine Internal medicine medicine biology.protein Immunology and Allergy Interferon gamma Interleukin 4 medicine.drug |
| Zdroj: | Clinical & Experimental Allergy. 26:349-356 |
| ISSN: | 0954-7894 |
| DOI: | 10.1111/j.1365-2222.1996.tb00102.x |
| Popis: | Summary Background Previous studies have shown cockroach-induced antigen-specific IgF-mediated asthma. In cockroach-Infested areas, more then 50% of asthmatic subjects may have positive skin reactions to this allergen. Partial purified Cr-PI allergen from American cockroaches contains allergens with molecular weights of 72 and 78k Da; however, little is known about its eifect on the lymphoeyte proliferation and cytokine production. Objective IgE synthesis is known to be regulated by interleukin-4 (IL-4) and interferon gamma (IFN-γ). Therefore, we studied Cr-PI allergen-induced cytokine production in atopic patients and healthy normal controls to understand each factors’ role in the disease. Methods Peripheral blood mononuclear cells (PBMC) from cockroach skin-sensitive patients and controls were stimulated with mitogen and Cr-PI for proliferative response and cytokine production. Cr-PI antigen-specific T-cell cultures of atopic patients and healthy normal controls were used to test C r-PI-lnduced proliferation and cytokine mKNA expression. Results PMBC ot atopic subjects showed a significantly (P < 0.01) higher stimulation index for Cr-PI induced proliferation (SI = l l.8.3.7) when compared with that of non-atopic subjects (SI =4.1 ± 0.8) and cord bloods (SI = 2.1 ± 0.4). Cr-Pl-induced IL-4 was observed only in the PBMC of atopic patients, whereas Cr-PI-induced IFNγ was detected in both atopic patients and normal controls. Likewise, Cr-PI-induced IL-4 mRNA expression in T-cell cultures was detected in all atopies but only one of nine controls. Conclusion IL-4 mKNA expression and IL-4 production in PBMC and T-cell cultures of atopic patients showed good correlation with clinical symptoms, skin-reactivity, specific IgE and proliferative response to Cr-PL These results suggests that cockroach allergen may be a hidden cause of asthma and other atopie diseases. |
| Databáze: | OpenAIRE |
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