High uptake carboxyl-functionalized porous β-cyclodextrin polymer for selective extraction of lysozyme from egg white
| Autor: | Zhi-Qi Zhang, Jun Wang, Hui-Ling Duan, Qi-Le Niu, Jing Zhang, Shi-Yao Ma |
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| Rok vydání: | 2019 |
| Předmět: |
Nitrile
Carboxylic Acids 010402 general chemistry 01 natural sciences Biochemistry Chemistry Techniques Analytical Analytical Chemistry Hydrolysis chemistry.chemical_compound Adsorption Egg White chemistry.chemical_classification Cyclodextrins Chromatography Cyclodextrin Chemistry beta-Cyclodextrins 010401 analytical chemistry Organic Chemistry General Medicine Polymer 0104 chemical sciences Chemical engineering Muramidase Lysozyme Selectivity Porosity Egg white |
| Zdroj: | Journal of Chromatography A. 1600:80-86 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2019.04.056 |
| Popis: | Lysozyme is widely used in medical, food and industrial fields due to its bacteriolytic effect and thus it is significant to design and develop a specific adsorbent with high adsorption capacity and selectivity for lysozyme. Inspired by the high uptake capacity of tetrafluoroterephthalonitrile-crosslinked porous β-cyclodextrin polymers (P-CDPs) and the noncovalent interaction between lysozyme and carboxyl groups, the carboxyl-functionalized P-CDPs (P-CDP-COO−) were synthesized by base-catalysed hydrolysis of nitrile group in P-CDPs to carboxyl. Porous structure, large extent of carboxylic functional groups, cyclodextrin's preventing aggregation, and negatively charged make P-CDP-COO− possess an outstanding adsorption capability for lysozyme. The maximum saturated adsorption capacity reaches 1520 mg g−1, which is much better than the parent polymer and other reported materials. The as-prepared material was successfully utilized for the selective extraction of lysozyme from egg white. |
| Databáze: | OpenAIRE |
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