Identification des progéniteurs bipotents qui donnent naissance aux tissus myogéniques et conjonctifs chez la souris
| Autor: | Alexandre Grimaldi, Shahragim Tajbakhsh, Glenda Comai, Sébastien Mella |
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| Přispěvatelé: | Cellules Souches et Développement / Stem Cells and Development, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), We acknowledge funding support from the Institut Pasteur, Association Française contre le Myopathies, Agence Nationale de la Recherche (Laboratoire d’Excellence Revive, Investissement d’Avenir, ANR-10-LABX-73) and MyoHead, Association Française contre les Myopathies (Grant #20510), Fondation pour la Recherche Médicale (Grant # FDT201904008277), and the Centre National de la Recherche Scientifique. We gratefully acknowledge the UtechS Photonic BioImaging, C2RT, Institut Pasteur, supported by the French National Research Agency (France BioImaging, ANR-10–INSB–04, Investments for the Future)., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
Cell type
Mesoderm [SDV]Life Sciences [q-bio] Cell Connective tissue regenerative medicine Biology Muscle Development General Biochemistry Genetics and Molecular Biology cranial muscles 03 medical and health sciences developmental biology Mice 0302 clinical medicine stem cells medicine Animals Progenitor cell Muscle Skeletal mouse 030304 developmental biology 0303 health sciences General Immunology and Microbiology General Neuroscience Neural crest Skeletal muscle Gene Expression Regulation Developmental [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis Cell Differentiation General Medicine scRNAseq Cell biology medicine.anatomical_structure [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis Connective Tissue Neural Crest cranial mesoderm MYF5 myogenesis 030217 neurology & neurosurgery |
| Zdroj: | eLife eLife, 2022, 11:e70235, ⟨10.7554/eLife.70235⟩ |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.70235⟩ |
| Popis: | How distinct cell fates are manifested by direct lineage ancestry from bipotent progenitors, or by specification of individual cell types is a key question for understanding the emergence of tissues. The interplay between skeletal muscle progenitors and associated connective tissue cells provides a model for examining how muscle functional units are established. Most craniofacial structures originate from the vertebrate-specific neural crest cells except in the dorsal portion of the head, where they arise from cranial mesoderm. Here, using multiple lineage-tracing strategies combined with single cell RNAseq and in situ analyses, we identify bipotent progenitors expressing Myf5 (an upstream regulator of myogenic fate) that give rise to both muscle and juxtaposed connective tissue. Following this bifurcation, muscle and connective tissue cells retain complementary signalling features and maintain spatial proximity. Disrupting myogenic identity shifts muscle progenitors to a connective tissue fate. The emergence of Myf5-derived connective tissue is associated with the activity of several transcription factors, including Foxp2. Interestingly, this unexpected bifurcation in cell fate was not observed in craniofacial regions that are colonised by neural crest cells. Therefore, we propose that an ancestral bi-fated program gives rise to muscle and connective tissue cells in skeletal muscles that are deprived of neural crest cells. |
| Databáze: | OpenAIRE |
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