Exploiting the Catalytic Ability of Polydopamine-Remodeling Gold Nanoparticles toward the Naked-Eye Detection of Cancer Cells at a Single-Cell Level
| Autor: | Bo-Zhao Yang, Zheng-Yuan Su, Amily Fang-Ju Jou |
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| Rok vydání: | 2021 |
| Předmět: |
Indoles
Polymers Biomedical Engineering Metal Nanoparticles Nanoprobe Nanoparticle Biocompatible Materials Breast Neoplasms Nanotechnology Biosensing Techniques Catalysis Biomaterials Materials Testing medicine Animals Humans Particle Size Cells Cultured Molecular Structure Chemistry Biochemistry (medical) RNA-Binding Proteins Cancer Serum Albumin Bovine General Chemistry Aptamers Nucleotide Phosphoproteins medicine.disease Oligodeoxyribonucleotides Colloidal gold Cancer cell Cattle Gold Naked eye Single-Cell Analysis Nucleolin |
| Zdroj: | ACS Applied Bio Materials. 4:2821-2828 |
| ISSN: | 2576-6422 |
| DOI: | 10.1021/acsabm.1c00041 |
| Popis: | In this study, a catalytic polydopamine-remodeling gold nanoparticle sensitized with an antinucleolin AS1411 probe (pAu nanoprobe) is synthesized, where the surface of the gold nanoparticles (AuNPs) is modified with a spontaneous self-polymerization of a polydopamine coating that imparts the probe functionalization ability and antispecific protein binding while the intrinsic catalytic property of the AuNPs is preserved. The functionalized AS1411 probe exerts specific recognition with nucleolin protein that is found to be overexpressed on the surface of breast cancer cells (MDA-MB-231). Scanning electron microscopy (SEM) confirms that the specific binding of the pAu nanoprobe occurs at the cancer cell surface. Taking advantage of the catalytic ability of the pAu nanoprobe in reducing blue-colored methylene blue (MB) to colorless leuco-MB, a colorimetric biosensing platform is established based on the accessible catalytic active sites on the pAu nanoprobe toward MB. The specific binding inhibits the pAu nanoprobe from efficiently catalyzing the reduction of MB, resulting in a " |
| Databáze: | OpenAIRE |
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