Brucella abortus-Stimulated Platelets Activate Brain Microvascular Endothelial Cells Increasing Cell Transmigration through the Erk1/2 Pathway
| Autor: | M. Victoria Delpino, Kwang Sik Kim, M. Cruz Miraglia, Agustina P. Melnyczajko, Aldana Trotta, Ana M. Rodríguez, Paula Barrionuevo, Guillermo H. Giambartolomei |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) CIENCIAS MÉDICAS Y DE LA SALUD Cell Inmunología BRUCELLA ABORTUS Brucella abortus lcsh:Medicine Article Endothelial activation neurobrucellosis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine Immunology and Allergy Secretion Platelet Molecular Biology CD40 General Immunology and Microbiology biology Chemistry lcsh:R BRAIN MICROVASCULAR ENDOTHELIAL CELLS purl.org/becyt/ford/3.1 [https] NEUROBRUCELLOSIS PLATELETS endothelial cells Cell biology Endothelial stem cell Medicina Básica 030104 developmental biology Infectious Diseases medicine.anatomical_structure platelets biology.protein purl.org/becyt/ford/3 [https] Bacterial outer membrane brain microvascular endothelial cells 030215 immunology |
| Zdroj: | Pathogens, Vol 9, Iss 708, p 708 (2020) Pathogens Volume 9 Issue 9 CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2076-0817 |
| Popis: | Central nervous system invasion by bacteria of the genus Brucella results in an inflammatory disorder called neurobrucellosis. A common feature associated with this pathology is blood&ndash brain barrier (BBB) activation. However, the underlying mechanisms involved with such BBB activation remain unknown. The aim of this work was to investigate the role of Brucella abortus-stimulated platelets on human brain microvascular endothelial cell (HBMEC) activation. Platelets enhanced HBMEC activation in response to B. abortus infection. Furthermore, supernatants from B. abortus-stimulated platelets also activated brain endothelial cells, inducing increased secretion of IL-6, IL-8, CCL-2 as well as ICAM-1 and CD40 upregulation on HBMEC compared with supernatants from unstimulated platelets. Outer membrane protein 19, a B. abortus lipoprotein, recapitulated B. abortus-mediated activation of HBMECs by platelets. In addition, supernatants from B. abortus-activated platelets promoted transendothelial migration of neutrophils and monocytes. Finally, using a pharmacological inhibitor, we demonstrated that the Erk1/2 pathway is involved in the endothelial activation induced by B. abortus-stimulated platelets and also in transendothelial migration of neutrophils. These results describe a mechanism whereby B. abortus-stimulated platelets induce endothelial cell activation, promoting neutrophils and monocytes to traverse the BBB probably contributing to the inflammatory pathology of neurobrucellosis. |
| Databáze: | OpenAIRE |
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