Direct reuse of electronic plastic scraps from computer monitor and keyboard to direct stem cell growth and differentiation

Autor: Jean-Christophe P. Gabriel, Jong-Min Lee, Zhuoran Wu, Chor Yong Tay, Pujiang Shi, Madhavi Srinivasan, Chiew Kei Tan
Přispěvatelé: Nanyang Technological University [Singapour], Laboratoire Innovation en Chimie des Surfaces et NanoSciences (LICSEN UMR 3685), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), School of Chemical & Biomedical Engineering, National Research Foundation, Singapore, and National Environment Agency, Singapore under its Closing the Waste Loop Funding Initiative (Award No. USS-IF-2018-4), Project 'NTU Singapore –CEA Alliance for Research in Circular Economy (SCARCE) - Research Thrust 4', Nanyang Technological University, Energy Research Institute at NTU (ERIAN), Laboratoire Innovation en Chimie des Surfaces et NanoSciences (LICSEN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), School of Materials Science and Engineering, School of Biological Sciences, 'NTU Singapore –CEA Alliance for Research in Circular Economy (SCARCE)', School of Chemical and Biomedical Engineering, Energy Research Institute @ NTU (ERI@N)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Environmental Engineering
business.product_category
Materials science
Waste Valorization
Cellular differentiation
Nanotechnology
02 engineering and technology
Substrate (printing)
Reuse
010402 general chemistry
01 natural sciences
Contact guidance
12. Responsible consumption
chemistry.chemical_compound
Osteogenesis
Humans
Environmental Chemistry
Diffuser (sewage)
Waste Management and Disposal
Waste management
Materials [Engineering]
Computers
Acrylonitrile butadiene styrene
Stem Cells
Cell Differentiation
[CHIM.MATE]Chemical Sciences/Material chemistry
021001 nanoscience & nanotechnology
Pollution
Waste valorization
0104 chemical sciences
Electronic-plastics
chemistry
Stem cell differentiation
Cell culture
Electronics
Computer monitor
Stem cell
0210 nano-technology
business
Plastics
Electronic-Plastics
Zdroj: Science of the Total Environment
Science of the Total Environment, 2022, 807 (3), pp.151085. ⟨10.1016/j.scitotenv.2021.151085⟩
Science of the Total Environment, Elsevier, In press, 2021 (807), pp.151085. ⟨10.1016/j.scitotenv.2021.151085⟩
ISSN: 0048-9697
1879-1026
Popis: Reuse of electronic wastes is a critical aspect for a more sustainable circular economy as it provides the simplest and most direct route to extend the lifespan of non-renewable resources. Herein, the distinctive surface and micro topographical features of computer electronic-plastic (E-plastic) scraps were unconventionally repurposed as a substrate material to guide the growth and differentiation of human adipose-derived mesenchymal stem cells (ADSCs). Specifically, the E-plastics were scavenged from discarded computer components such as light diffuser plate (polyacrylates), prismatic sheet (polyethylene terephthalate), and keyboards (acrylonitrile butadiene styrene) were cleaned, sterilized, and systematically characterized to determine the identity of the plastics, chemical constituents, surface features, and leaching characteristics. Multiparametric analysis revealed that all the E-plastics could preserve stem-cell phenotype and maintain cell growth over 2 weeks, rivalling the performance of commercial tissue-culture treated plates as cell culture plastics. Interestingly, compared to commercial tissue-culture treated plastics and in a competitive adipogenic and osteogenic differentiation environment, ADSCs cultured on the keyboard and light diffuser plastics favoured bone cells formation while the grating-like microstructures of the prismatic sheet promoted fat cells differentiation via the process of contact guidance. Our findings point to the real possibility of utilizing discarded computer plastics as a "waste-to-resource" material to programme stem cell fate without further processing nor biochemical modification, thus providing an innovative second-life option for E-plastics from personal computers. National Environmental Agency (NEA) National Research Foundation (NRF) This research is supported by the National Research Foundation, Singapore, and National Environment Agency, Singapore under its Closing the Waste Loop Funding Initiative (Award No. USS-IF-2018-4).
Databáze: OpenAIRE
Externí odkaz: