Migration of CD18-deficient neutrophils in vitro: evidence for a CD18-independent pathway induced by IL-8
| Autor: | Bruce J Morland, Phil J Darbyshire, Robert A. Stockley, Clare M Morland |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Neutrophils Leukocyte-Adhesion Deficiency Syndrome Integrin alphaXbeta2 Macrophage-1 Antigen CD18 Antibodies Cell Movement Cell Adhesion medicine Humans Interleukin 8 Molecular Biology Cells Cultured Membrane Glycoproteins CD11 Antigens Chemistry Cell adhesion molecule Leukocyte adhesion deficiency-1 Chemotaxis Interleukin-8 Neutrophil Soluble cell adhesion molecules Infant Flow Cytometry medicine.disease Precipitin Tests Lymphocyte Function-Associated Antigen-1 In vitro Cell biology Endothelial stem cell Neutrophil Infiltration CD18 Antigens Adhesion Diffusion Chambers Culture Molecular Medicine Endothelium Vascular |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1500(1):70-76 |
| ISSN: | 0925-4439 |
| DOI: | 10.1016/s0925-4439(99)00089-7 |
| Popis: | Neutrophils isolated from a child with severe leukocyte adhesion deficiency 1 (LAD1) had a complete absence of expression of the CD11/CD18 β2 integrin family of adhesion molecules, and were shown to be deficient in the in vitro adhesion and migration properties. However, we found that interleukin-8 (IL8), a potent chemoattractant for neutrophils, and sputum sol phase induced these LAD1 neutrophils to migrate through an endothelial cell layer in vitro, and confirmed that this migration was CD18-independent. These findings add to evidence of CD18-independent mechanisms of neutrophil recruitment, in particular neutrophil infiltration into the lungs, where IL8 may be an important recruitment factor. |
| Databáze: | OpenAIRE |
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