Role of ubiquitin-protein ligase UBR5 in the disassembly of mitotic checkpoint complexes

Autor:	Sharon Kaisari, Shirly Miniowitz-Shemtov, Danielle Sitry-Shevah, Pnina Shomer, Guennadi Kozlov, Kalle Gehring, Avram Hershko
Rok vydání:	2022
Předmět:	Multidisciplinary Ubiquitin-Protein Ligases Ubiquitination Humans M Phase Cell Cycle Checkpoints Mitosis food and beverages Cell Cycle Proteins Poly-ADP-Ribose Binding Proteins Protein Binding
Zdroj:	Proceedings of the National Academy of Sciences. 119
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.2121478119
Popis:	Significance The mitotic checkpoint system is essential for the prevention of mistakes in the segregation of chromosomes in mitosis. As long as chromosomes are not attached correctly to the mitotic spindle, a mitotic checkpoint complex (MCC) is assembled and inhibits the action of ubiquitin ligase APC/C (anaphase-promoting complex/cyclosome) to initiate anaphase. When the checkpoint is turned off, MCC is disassembled, allowing anaphase initiation. The mechanisms of MCC disassembly have been studied, but the regulation of this process remained obscure. We found that a second ubiquitin ligase, UBR5 (ubiquitin-protein ligase N -recognin 5), ubiquitylates MCC components and stimulates the disassembly of MCC from APC/C, as well as the dissociation of a subcomplex of MCC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::092ee69c5a2863319f1f9a797ec450a1 https://doi.org/10.1073/pnas.2121478119 Zobrazit plný text záznamu