Author Correction: Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling
| Autor: | María Virumbrales-Muñoz, Sara Oliván, Teodora Randelovic, Desirée Pereboom, Jose M. Ayuso, Alodia Lacueva, Luis J. Fernández, Ignacio Ochoa, Megan K. Livingston, David J. Beebe, Manuel Doblaré |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Cells Microfluidics Cell Cell Culture Techniques lcsh:Medicine Cell Line 03 medical and health sciences 3D cell culture 0302 clinical medicine Tumor Microenvironment medicine Humans Profiling (information science) Collagenases Author Correction lcsh:Science 030304 developmental biology 0303 health sciences Multidisciplinary Chemistry lcsh:R Hydrogels medicine.anatomical_structure Cancer research lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-1 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-57830-0 |
| Popis: | The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Microfluidics can be used to reproduce the TME in vitro and hence provide valuable insight on tumour evolution and drug sensitivity. However, microfluidics remains far from well-established mainstream molecular and cell biology methods. Therefore, we have developed a quick and straightforward collagenase-based enzymatic method to recover cells embedded in a 3D hydrogel in a microfluidic device with no impact on cell viability. We demonstrate the validity of this method on two different cell lines in a TME microfluidic model. Cells were successfully retrieved with high viability, and we characterised the different cell death mechanisms via AMNIS image cytometry in our model. |
| Databáze: | OpenAIRE |
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