Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
| Autor: | Claudia Balagué, Cecilia Casabonne, Virginia Aquili, Andrea del Lujan Quiberoni, David Damian Tomat, Marina del Valle Soazo, Roxana Andrea Verdini |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Whey protein CIENCIAS MÉDICAS Y DE LA SALUD EDIBLE FILM Biotecnología relacionada con la Salud Active packaging Liquid medium medicine.disease_cause 01 natural sciences Biotecnología de la Salud Matrix (chemical analysis) 0404 agricultural biotechnology 010608 biotechnology medicine Food science purl.org/becyt/ford/3.4 [https] Escherichia coli Chemistry food and beverages 04 agricultural and veterinary sciences WHEY PROTEIN CONCENTRATE Antimicrobial 040401 food science PHAGE COCKTAIL Lytic cycle Solid food purl.org/becyt/ford/3 [https] ESCHERICHIA COLI Food Science |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted in the whey protein concentrate (WPC) matrix over 5 weeks of storage. In addition, the phages incorporated in the WPC matrix were characterized by their release from films into an aqueous system and solid food, as well as by their antimicrobial activity against pathogenic E. coli in a liquid medium and in meat at refrigerated, room and abusive temperatures. The results demonstrate that phages are highly stable in WPC films within 5 weeks of storage. Moreover, WPC films can release a significant number of phages into an aqueous system and onto a meat surface at similar concentrations. Phage-added films produced a significant reduction in E. coli cells to non-detectable levels in antimicrobial assays. Similar results, such as a complete inactivation achieved for DH5α and O157:H7 STEC strains, were obtained when antimicrobial assays were performed on meat. As a conclusion, the six-phage cocktail added into WPC films was highly stable, effectively released from films and proved highly effective as a biocontrol tool, though not under all the conditions evaluated. Fil: Tomat, David Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Soazo, Marina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Verdini, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Casabonne, Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Aquili, Virginia Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina |
| Databáze: | OpenAIRE |
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