Synaptonemal complex components are required for meiotic checkpoint function in C. elegans
| Autor: | Kyra Firestone, Evan Eggleston, Tisha Bohr, Needhi Bhalla, Guinevere Ashley |
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| Rok vydání: | 2016 |
| Předmět: |
Genetics
0303 health sciences Cell cycle checkpoint Mutant Synapsis Meiotic chromosome segregation Biology 3. Good health 03 medical and health sciences Synaptonemal complex 0302 clinical medicine Meiosis Pairing Homologous chromosome biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/052977 |
| Popis: | Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes and is essential for proper meiotic chromosome segregation. In C. elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on Pairing Centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3 and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bonafide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint. |
| Databáze: | OpenAIRE |
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