Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
| Autor: | Stephan Deimel, Javier Avalos, Ulrich Terpitz, Jorge García-Martínez, M. Carmen Limón, Javier Pardo-Medina, Alexander Adam, Tilen Konte |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Genética, Ministerio de Ciencia y Tecnología (MCYT). España, Junta de Andalucía |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sodium Acetate genetic structures Sequence Homology 01 natural sciences lcsh:Chemistry Fusarium CarO udc:577 lcsh:QH301-705.5 Spectroscopy Fungal protein biology General Medicine Proton Pumps patch-clamp Computer Science Applications Biochemistry Rhodopsin Host-Pathogen Interactions Bakanae bakanae Plant hormone Fusarium fujikuroi fungal rhodopsins OpsA Oryza sativa rice–plant infection green light perception indole-3-acetic acid (IAA) Fungus Neurospora Catalysis Article Inorganic Chemistry Fungal Proteins 03 medical and health sciences ddc:570 Physical and Theoretical Chemistry Molecular Biology indol-3-ocetna kislina (IAA) Indoleacetic Acids protein activity Organic Chemistry fungi Oryza glivični rodopsini biology.organism_classification 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 biology.protein sense organs aktivnost beljakovin 010606 plant biology & botany |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 215 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 1, p 215 (2018) International journal of molecular sciences, vol. 19, no. 1, 215, 2018. |
| ISSN: | 1422-0067 |
| Popis: | Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus. España Ministerio de Ciencia y tecnología BIO20010-15430 Junta de Andalucía CTS-6638 |
| Databáze: | OpenAIRE |
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