Antibodies to Respiratory Syncytial Virus Polypeptides and their Significance in Human Infection
| Autor: | P. J. Watt, K. A. Ward, M. M. Ogilvie, P. R. Lambden |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Paramyxoviridae
medicine.drug_class medicine.medical_treatment Radioimmunoassay Passive immunity Biology Antibodies Viral Monoclonal antibody Respirovirus Infections Virus Mice Viral Proteins Antigen Virology medicine Animals Humans Antigens Viral Mice Inbred BALB C Antibodies Monoclonal Infant Radioimmunoprecipitation Assay biology.organism_classification Respiratory Syncytial Viruses Immunoglobulin G Immunology biology.protein Viral disease Antibody Peptides Immunity Maternally-Acquired |
| Zdroj: | Journal of General Virology. 64:1867-1876 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-64-9-1867 |
| Popis: | The human antibody response to respiratory syncytial (RS) virus infection was investigated using radioimmunoprecipitation analysis (RIPA). A total of nine RS virus-specific proteins, VP200, VGP95, VP68, VGP48, VPN41, VP35, VP27, VP23 and VGP20 were identified by comparing 35S- or 3H-labelled extracts of infected and uninfected HEp-2 cells, and by radioimmunoprecipitation using a hyperimmune human serum. Three glycopeptides, VGP95, VGP48 and VGP20, were identified by incorporation of [3H]glucosamine, and two of these (VGP48 and VGP20) were assumed to be part of a single disulphide-bonded polypeptide since they were precipitated by a monoclonal antibody raised against a surface protein. Human serum antibodies to three major RS virus proteins, VGP95, VGP48/VGP20 and VPN41 were measured by RIPA using radioiodinated RS virus antigens. Sera from a group of mothers whose babies escaped RS virus infection during a local epidemic showed increased antibody levels to VPN41 when compared to sera from mothers whose babies had become infected with RS virus within the first 6 months of life. In infants who remained uninfected with RS virus during the first 12 months of life the maternal gift of antibody decayed to about 50% at 3 months with traces of antibodies detected in a few sera at 12 months. The antibody levels detected in the sera of infants less than 3 months old convalescent from primary RS virus infection did not exceed the mean levels present in the serum of uninfected babies. Infants between the ages of 6 and 12 months were able to mount an IgG response to VPN41 and VGP48 but, unlike adults and older children, a particularly striking finding was their failure to produce antibodies to VGP95. |
| Databáze: | OpenAIRE |
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