The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS

Autor: Mikel Vicente Eceiza, Miriam Gil-Monreal, María Barco-Antoñanzas, Ana Zabalza, Mercedes Royuela
Přispěvatelé: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. IMAB - Institute for Multidisciplinary Research in Applied Biology, Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Rok vydání: 2022
Předmět:
Zdroj: Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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ISSN: 0176-1617
Popis: The present study aimed to determine whether glyphosate-induced oxidative stress is directly related to the action mechanism of this herbicide (5-enolpyruvylshikimate-3-phosphate synthase or EPSPS inhibition) and analyse the role of oxidative stress in glyphosate toxicity of the weed Amaranthus palmeri S. Wats. Two kinds of populations were studied using EPSPS amplification: glyphosate-sensitive and glyphosate-resistant (by gene amplification). Plants were grown hydroponically and treated with different glyphosate doses, after which several oxidative stress markers were measured in the leaves. Untreated, sensitive and resistant plants showed similar values for the analysed parameters. Treated glyphosate-sensitive plants showed an increase in shikimate, superoxide and H2O2 contents and dose-dependent lipid peroxidation and antioxidant responses; however, none of these effects were observed in resistant plants, indicating that glyphosate-induced oxidative stress is related to EPSPS inhibition. Oxidative stress is associated with an increase in the activity of peroxidases due to EPSPS inhibition, although the link between both processes remains elusive. The fact that some glyphosate doses were lethal but did not induce major oxidative damage provides evidence that glyphosate toxicity is independent of oxidative stress. This work was funded by the Spanish Ministry of Economy and Competitiveness (AGL2016-77531-R), the Public University of Navarre (Project UPNA20-6138) and the Spanish Ministry of Science and Innovation (2020-117723RB-100). M.V. Eceiza is the holder of a predoctoral fellowship of the Basque Government. Open access funding provided by Universidad Pública de Navarra.
Databáze: OpenAIRE
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