Modulation of epidermal growth factor receptors by human alpha interferon
| Autor: | Renqiu Hu, D. L. Zur Nedden, Kathryn C. Zoon, Heinz Arnheiter, Y. Karasaki |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
media_common.quotation_subject Alpha interferon Biology Interferon-gamma Interferon Epidermal growth factor Internal medicine medicine Humans Receptor Internalization Cells Cultured Interferon alfa media_common Multidisciplinary Epidermal Growth Factor Cell growth Molecular biology ErbB Receptors Endocrinology Exposure period Interferon Type I Cell Division hormones hormone substitutes and hormone antagonists Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 83:8226-8230 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.83.21.8226 |
| Popis: | Treatment of Madin-Darby bovine kidney (MDBK) cells with human interferon (IFN)-alpha 2 at 37 degrees C results in a dose-dependent inhibition of cell growth and a reduction of the subsequent binding of 125I-labeled epidermal growth factor (EGF) at 4 degrees C. Human IFN-beta and -gamma, which exhibit little antiviral and antiproliferative activities on MDBK cells, have little effect on cell growth or the binding of 125I-labeled EGF to these cells. The binding of EGF is decreased after exposure to IFN-alpha for greater than 8 hr. Scatchard analyses of the EGF binding data indicate that a 20-hr exposure period results in a decrease in the apparent number of cell-surface EGF receptors and a reduction in the affinity of EGF for its receptor. The rate of internalization of EGF by MDBK cells does not appear to be affected by IFN treatment. |
| Databáze: | OpenAIRE |
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