Chronic effects of carbamazepine on zebrafish: Behavioral, reproductive and biochemical endpoints
| Autor: | Níchollas Serafim Camargo, Miguel Oliveira, Vitória Perillo, Niedja da Silva Santos, Joana Mona e Pinto, Carolina Almeida Lisboa, Rhaul Oliveira, Diego Sousa-Moura, Cesar Koppe Grisolia, Inês Domingues |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Gills
Male 0301 basic medicine Gill medicine.medical_specialty Antioxidant Aché Health Toxicology and Mutagenesis medicine.medical_treatment 010501 environmental sciences 01 natural sciences Antioxidants 03 medical and health sciences chemistry.chemical_compound Lactate dehydrogenase Internal medicine medicine Animals Zebrafish Glutathione Transferase 0105 earth and related environmental sciences Micronucleus Tests biology Reproduction Public Health Environmental and Occupational Health Feeding Behavior General Medicine Glutathione Metabolism Hydrogen-Ion Concentration Catalase Pollution Acetylcholinesterase language.human_language Oxidative Stress Carbamazepine 030104 developmental biology Endocrinology Liver chemistry language biology.protein Female Water Pollutants Chemical |
| Zdroj: | Ecotoxicology and Environmental Safety. 164:297-304 |
| ISSN: | 0147-6513 |
| DOI: | 10.1016/j.ecoenv.2018.08.015 |
| Popis: | Carbamazepine (Cbz), one of the most prescribed pharmaceuticals in the world is often detected in surface waters and sediments. However, few studies addressed its chronic effects in fish. In the present study, Danio rerio adults were exposed for 63 days to Cbz (0 - control, 10 μg L−1 - concentration found in effluents, and 10,000 μg L−1 − 5% of LC50 at 72 h). Assessed endpoints were: feeding behavior, growth rate, number of eggs produced and their viability, histological alterations in female gonads, and biochemical biomarkers associated with antioxidant defenses (catalase - CAT, and glutathione S-transferase – GST activities), neurotransmission (acetylcholinesterase activity - AChE) and metabolism (lactate dehydrogenase - LDH). Cbz exposure increased the total time for food intake but did not affect D. rerio growth. Although the total number of eggs was not affected by Cbz exposure, the eggs viability was significantly impaired. Exposure to Cbz caused alterations in the female gonads follicular stages. In terms of biochemical endpoints, CAT activity in liver and gills, was sensitive to the pharmaceutical exposure presenting a decreased activity. AChE activity was induced in the head (both concentrations) and muscle (10,000 μg L−1). GST activity was increased in gills (both concentrations) but inhibited in the intestine. Concerning LDH, enzymatic activity was increased in the liver and decreased in muscle and gills. Several of the above-mentioned effects can be directly linked with effects at population level (e.g. feeding behavior) and occurred at environmental concentrations (the lowest concentration tested), thus serious concerns regarding risks posed by Cbz residues to fish populations arise with this study. |
| Databáze: | OpenAIRE |
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