In situ detection of antigen-specific T cells in cryo-sections using MHC class I tetramers after dendritic cell vaccination of melanoma patients

Autor: Cornelis J. A. Punt, M. R. Bernsen, Dirk J. Ruiter, Gosse J. Adema, Carl G. Figdor, I.J.M. de Vries, G.N.P. van Muijen, P. D. M. Rombout, Nicole M. Scharenborg, W.J. Lesterhuis, W. L. van Geloof
Přispěvatelé: Other departments
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Cancer Research
Skin Neoplasms
T cell
CD8 Antigens
Immunology
CD1
chemical and pharmacologic phenomena
Neuroinformatics [DCN 3]
Biology
Mice
Immune system
Immune Regulation [NCMLS 2]
Translational research [ONCOL 3]
Antigens
Neoplasm
MHC class I
Influenza
Human
medicine
Immunology and Allergy
Cytotoxic T cell
Animals
Humans
Hypersensitivity
Delayed
Melanoma
Molecular diagnosis
prognosis and monitoring [UMCN 1.2]
Hereditary cancer and cancer-related syndromes [ONCOL 1]
Staining and Labeling
Histocompatibility Antigens Class I
Vaccination
Immunotherapy
gene therapy and transplantation [UMCN 1.4]
Dendritic cell
Dendritic Cells
130 030 The schema-consolidation hypothesis
Molecular biology
Pathogenesis and modulation of inflammation [N4i 1]
CTL
Disease Models
Animal
medicine.anatomical_structure
Oncology
biology.protein
Clone (B-cell biology)
Functional Neurogenomics [DCN 2]
Cryoultramicrotomy
Immunity
infection and tissue repair [NCMLS 1]
T-Lymphocytes
Cytotoxic
Zdroj: BASE-Bielefeld Academic Search Engine
Cancer Immunology Immunotherapy, 56, 1667-76
Cancer immunology, immunotherapy, 56(10), 1667-1676. Springer Science and Business Media Deutschland GmbH
Cancer Immunology Immunotherapy, 56, 10, pp. 1667-76
Monique Bernsen
ISSN: 0340-7004
Popis: Contains fulltext : 53002.pdf (Publisher’s version ) (Closed access) Application of tetrameric MHC class I-peptide complexes has significantly improved the monitoring of antigen-specific T cell immune responses in mouse models as well as in clinical studies. Especially MHC class I tetramer analysis of tumor-specific T cells in suspension or on thick vibratome sections from viable tissue has been proven extremely useful. Using the well-characterized mouse tyrosinase-related-protein-2 specific cytotoxic T cell (CTL) clone LP9, we now developed a method that allows for specific identification of T cells with MHC class I tetramers in 8 mum thick, chemically fixed cryosections. The protocol was validated in a murine influenza virus-infection model. Moreover, analysis of delayed type hypersensitivity (DTH) skin biopsies from melanoma patients vaccinated with peptide-loaded mature dendritic cells, revealed the presence and location of anti-tumor CTLs. The specificity of the CTLs detected in situ correlated with both the DTH challenge specificity and reactivity of cell suspensions derived from the same biopsies. Collectively, our data demonstrate that in situ MHC class I tetramer staining provides a valuable tool to reveal the presence and anatomical location of specific CTLs in frozen tissue following immune-based treatment strategies in cancer patients.
Databáze: OpenAIRE
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