Matrix metalloproteinases in human choroidal neovascular membranes excised following verteporfin photodynamic therapy
| Autor: | Salvatore Grisanti, Gabor B. Scharioth, Kei Shinoda, Karl Ulrich Bartz-Schmidt, Efdal Yoeruek, Annemarie Adam, Micheal Klein, Olcay Tatar, Tillmann Eckert |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Porphyrins genetic structures Angiogenesis medicine.medical_treatment Photodynamic therapy Immunoenzyme Techniques Neovascularization Macular Degeneration Cellular and Molecular Neuroscience Stroma medicine Humans Aged Retrospective Studies Aged 80 and over Photosensitizing Agents business.industry Verteporfin Middle Aged Macular degeneration medicine.disease Choroidal Neovascularization eye diseases Sensory Systems Endostatins Extended Report Ophthalmology Choroidal neovascularization Matrix Metalloproteinase 9 Photochemotherapy Matrix Metalloproteinase 2 Female sense organs medicine.symptom Endostatin business Biomarkers medicine.drug |
| Zdroj: | British Journal of Ophthalmology. 91:1183-1189 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.2007.114769 |
| Popis: | To evaluate expression of proangiogenic matrix metalloproteinases (MMP) 2 and 9 at distinct intervals after verteporfin photodynamic therapy (PDT) in human choroidal neovascular membranes (CNV) secondary to age-related macular degeneration (AMD).Retrospective review of an interventional case series of 49 patients who underwent removal of CNV. Twenty-six patients were treated with PDT 3 to 383 days prior to surgery. Twenty-three CNV without previous treatment were used as controls. CNV were stained for CD34, cytokeratin 18, endostatin, MMP-2 and MMP-9 by immunohistochemistry.CNV without previous therapy disclosed MMP-2, MMP-9 in RPE-Bruch's membrane, vessels and stroma in different intensities. Three days after PDT, MMP-9 expression was significantly weaker in stroma (p = 0.0019). Endostatin was significantly reduced in vessels (p0.001). At longer post-PDT intervals, a significant increase of MMP-9 in stroma (p = 0.037) and of endostatin in RPE-Bruch's membrane (p = 0.02), vessels (p = 0.005) and stroma (p0.001) were disclosed. No significant changes in MMP-2 expression were detected.PDT induced an early, temporary decrease in MMP-9 and endostatin expression. At longer intervals, MMP-9 increase is possibly associated with the angiogenic process responsible for recurrence after PDT. MMP-9, however, acts as a double-edged sword by concomitant induction of endostatin, an endogenous inhibitor of angiogenesis. |
| Databáze: | OpenAIRE |
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