Reduced Vitellogenesis and Female Fertility in Gper Knockout Zebrafish
| Autor: | Yong Zhu, Peter Thomas, Aubrey Converse, Marcus Jermaul Williams, Kimberly A. Kew, Xin-Jun Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Ovulation
0301 basic medicine medicine.drug_class Endocrinology Diabetes and Metabolism Estrogen receptor 030209 endocrinology & metabolism oocyte growth lcsh:Diseases of the endocrine glands. Clinical endocrinology Receptors G-Protein-Coupled Andrology Vitellogenins 03 medical and health sciences Vitellogenin Follicle Endocrinology 0302 clinical medicine Ovarian Follicle G-protein coupled estrogen receptor (Gper) medicine Animals Metabolomics Ovarian follicle Zebrafish Original Research lcsh:RC648-665 biology Cell Membrane Vitellogenesis Fishes Estrogens Zebrafish Proteins biology.organism_classification ErbB Receptors Fertility 030104 developmental biology medicine.anatomical_structure Receptors Estrogen Estrogen Mutation Oocytes biology.protein Female reduced fertility vitellogenin GPER epidermal growth factor receptor (Egfr) |
| Zdroj: | Frontiers in Endocrinology, Vol 12 (2021) Frontiers in Endocrinology |
| ISSN: | 1664-2392 |
| Popis: | The role G-protein coupled estrogen receptor (GPER) plays in vertebrate reproduction remains controversial. To investigate GPER’s reproductive role, we generated a gper zebrafish mutant line (gper−/−) using TALENs. Gper mutant females exhibited reduced fertility with a 40.85% decrease in embryo production which was associated with a significant decrease in the number of Stage V (730–750 μm) ovulated oocytes. Correspondingly, the number of early vitellogenic follicles (Stage III, 400–450 µm) in gper−/− ovaries was greater than that in wildtypes (wt), suggesting that subsequent follicle development was retarded in the gper−/− fish. Moreover, plasma vitellogenin levels were decreased in gper−/− females, and epidermal growth factor receptor (Egfr) expression was lower in Stage III vitellogenic oocytes than in wt counterparts. However, hepatic nuclear estrogen receptor levels were not altered, and estrogen levels were elevated in ovarian follicles. These results suggest that Gper is involved in the control of ovarian follicle development via regulation of vitellogenesis and Egfr expression in zebrafish. |
| Databáze: | OpenAIRE |
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