The Isolated in Utero Environment Is Conducive to the Emergence of RNA and DNA Virus Variants
| Autor: | Uladzimir Karniychuk, Henry Munyanduki, Ivan Trus, Nathalie G. Bérubé, Daniel Udenze |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Circovirus
placenta Swine intra-host evolution viruses Microbiology Genome Article Virus Zika virus Genetic Heterogeneity viral evolution Virology Chlorocebus aethiops Animals Vero Cells biology Uterus Genetic Variation High-Throughput Nucleotide Sequencing porcine circovirus RNA DNA virus RNA virus Viral Load biology.organism_classification QR1-502 fetus Porcine circovirus Infectious Diseases Cellular Microenvironment Viral evolution Female pregnancy Directed Molecular Evolution |
| Zdroj: | Viruses, Vol 13, Iss 1827, p 1827 (2021) Viruses Volume 13 Issue 9 |
| ISSN: | 1999-4915 |
| Popis: | The host’s immune status may affect virus evolution. Little is known about how developing fetal and placental immune milieus affect virus heterogeneity. This knowledge will help us better understand intra-host virus evolution and how new virus variants emerge. The goal of our study was to find out whether the isolated in utero environment—an environment with specialized placental immunity and developing fetal immunity—supports the emergence of RNA and DNA virus variants. We used well-established porcine models for isolated Zika virus (RNA virus) and porcine circovirus 2 (DNA virus) fetal infections. We found that the isolated in utero environment was conducive to the emergence of RNA and DNA virus variants. Next-generation sequencing of nearly whole virus genomes and validated bioinformatics pipelines identified both unique and convergent single nucleotide variations in virus genomes isolated from different fetuses. Zika virus and PCV2 in utero evolution also resulted in single nucleotide variations previously reported in the human and porcine field samples. These findings should encourage further studies on virus evolution in placenta and fetuses, to better understand how virus variants emerge and how in utero viral evolution affects congenital virus transmission and pathogenicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |