p53 promotes inflammation-associated hepatocarcinogenesis by inducing HMGB1 release
| Autor: | Han Wu, He-Xin Yan, Hong-Ping Wu, Qi-Long Ying, Chang Tong, Qi-Jun Qian, Hongyang Wang, Hui-Lu Zhang, Charles Ashton |
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| Rok vydání: | 2013 |
| Předmět: |
Transcriptional Activation
Programmed cell death DNA damage Inflammation HMGB1 medicine.disease_cause Article Cell Line Proinflammatory cytokine Gene Knockout Techniques Liver Neoplasms Experimental medicine Animals Diethylnitrosamine HMGB1 Protein Carcinogen Hepatitis Chronic Hepatology biology Wild type Genes p53 Rats Cancer research biology.protein Inflammation Mediators Tumor Suppressor Protein p53 medicine.symptom Carcinogenesis |
| Zdroj: | Journal of Hepatology. 59:762-768 |
| ISSN: | 0168-8278 |
| Popis: | Background & Aims Hepatocellular carcinoma (HCC) develops in response to chronic hepatic injury. Although induced cell death is regarded as the major component of p53 tumor-suppressive activity, we recently found that sustained p53 activation subsequent to DNA damage promotes inflammation-associated hepatocarcinogenesis. Here we aim at exploring the mechanism linking p53 activation and hepatic inflammation during hepatocarcinogenesis. Methods p53 −/− hepatocytes expressing inducible p53 and primary wild type hepatocytes were treated to induce p53 expression. The supernatants were collected and analyzed for the presence of released inflammatory cytokines. Ethyl pyruvate was used in a rat model of carcinogen-induced hepatocarcinogenesis to examine its effect on p53-dependent chronic hepatic injury, inflammation, and tumorigenesis. Results Here we show that cytoplasmic translocation and circulating levels of potent inflammatory molecule high-mobility group protein 1 (HMGB1) were greater in wild type rats than in p53 +/− rats following carcinogen administration. Restoration of p53 expression in p53-null hepatocytes or induction of endogenous p53 in wild type hepatocytes gives rise to the release of HMGB1. Administration of the HMGB1 release inhibitor ethyl pyruvate, which does not affect p53-mediated hepatic apoptosis, substantially prevented carcinogen-induced cirrhosis and tumorigenesis in rat livers. Conclusions These results suggest that although p53 is usually regarded as a tumor suppressor, its constant activation can promote pro-tumorigenic inflammation, at least in part, via inducing HMGB1 release. Application of HMGB1 inhibitors when restoring p53 in cancer therapy might protect against pro-tumorigenic effects while leaving p53-mediated clearance of malignant cells intact. |
| Databáze: | OpenAIRE |
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