Integration of an optical CMOS sensor with a microfluidic channel allows a sensitive readout for biological assays in point-of-care tests
Autor: | Lieven J. Stuyver, Roelf Anco Jacob Groenhuis, Bieke Van Dorst, Simon Reuvekamp, Erik-Jan Lous, Adewole Adojutelegan, Simone Tanzi, Elfried van der Sar, Filip Frederix, Marko Blom, Monica Brivio |
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Rok vydání: | 2016 |
Předmět: |
Computer science
Point-of-care testing Microfluidics Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Antibodies Electrochemistry Humans Detection limit CMOS sensor business.industry 010401 analytical chemistry Proteins Oxides General Medicine Dipstick Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Photon counting 0104 chemical sciences Semiconductors CMOS Metals Point-of-Care Testing Biological Assay Colorimetry Peptides 0210 nano-technology business Sensitivity (electronics) Computer hardware Biotechnology |
Zdroj: | Biosensors and Bioelectronics. 78:126-131 |
ISSN: | 0956-5663 |
Popis: | In this manuscript, a microfluidic detection module, which allows a sensitive readout of biological assays in point-of-care (POC) tests, is presented. The proposed detection module consists of a microfluidic flow cell with an integrated Complementary Metal-Oxide-Semiconductor (CMOS)-based single photon counting optical sensor. Due to the integrated sensor-based readout, the detection module could be implemented as the core technology in stand-alone POC tests, for use in mobile or rural settings. The performance of the detection module was demonstrated in three assays: a peptide, a protein and an antibody detection assay. The antibody detection assay with readout in the detection module proved to be 7-fold more sensitive that the traditional colorimetric plate-based ELISA. The protein and peptide assay showed a lower limit of detection (LLOD) of 200 fM and 460 fM respectively. Results demonstrate that the sensitivity of the immunoassays is comparable with lab-based immunoassays and at least equal or better than current mainstream POC devices. This sensitive readout holds the potential to develop POC tests, which are able to detect low concentrations of biomarkers. This will broaden the diagnostic capabilities at the clinician's office and at patient's home, where currently only the less sensitive lateral flow and dipstick POC tests are implemented. |
Databáze: | OpenAIRE |
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