Optimal concentration of ammonium ion in a minimal synthetic medium for the growth of Candida albicans
| Autor: | Valerio Vidotto, Laura Guevara Ochoa, Massimo Bruatto, Juan Michea Cortes |
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| Rok vydání: | 1991 |
| Předmět: |
Chromatography
biology Strain (chemistry) Cell growth Veterinary (miscellaneous) biology.organism_classification Applied Microbiology and Biotechnology Microbiology Corpus albicans Yeast Culture Media Absorbance chemistry.chemical_compound Biotin chemistry Biochemistry Ammonium Sulfate Candida albicans Ammonium Agronomy and Crop Science |
| Zdroj: | Mycopathologia. 113(3) |
| ISSN: | 0301-486X |
| Popis: | Candida albicans strain B 311-10 with and without starvation was cultivated in the minimal synthetic medium of Shepherd et al. [18], modified without biotin, aminoacids, low glucose concentration [20] and with decreasing amounts of (NH4)2SO4, to determine the optimal growth requirement for this strain. All the experiments were carried out under sterile conditions at 25 °C in a thermostat with initial O.D.s (675 nm) of 0.500 and 0.100. Cell growth was generally monitored everyday for six days with a spectrophotometer by determining the absorbance of the cultures at 675 nm. All the experiments were repeated three times and a statistical analysis of the data with a probability of 99% and 1% of error was performed to confirm the validity of the results. Best growth was obtained with starved cells at an initial O.D. of 0.100 and with a 0.1 g/L concentration of (NH4)2SO4. At this concentration, the growth of C. albicans B 311-10 was best between the first and the fourth day with the maximum at the third day. With (NH4)2SO4 concentrations of 0.05 and 0.5 g/L, cell growth was the same. |
| Databáze: | OpenAIRE |
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