Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)
| Autor: | José Eduardo Serrão, Claudia Andrea Lima Cardoso, Denise Sguarizi Antonio, Pollyanna Pereira Santos, William Fernando Antonialli Junior, Rafaella C. Bernardi Marchiotti, Angélica Mendonça, Ellen Liciane Barbosa Firmino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Gel electrophoresis Apoica pallens biology Vespidae Mass spectrometry MALDI-TOF/TOF fungi Wasps 010607 zoology Proteins Venom Hymenoptera biology.organism_classification Proteomics 01 natural sciences complex mixtures 010602 entomology Sting Biochemistry QL1-991 Insect Science Zoology Apoica |
| Zdroj: | Revista Brasileira de Entomologia, Vol 63, Iss 4, Pp 322-330 (2020) Revista Brasileira de Entomologia v.63 n.4 2019 Revista brasileira de entomologia Sociedade Brasileira De Entomologia (SBE) instacron:SBE |
| ISSN: | 1806-9665 |
| Popis: | Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories: allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important. |
| Databáze: | OpenAIRE |
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