Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-Ras(V12) Oncogene
| Autor: | Gennaro Chiappetta, Michela Vitiello, Simona Camorani, Anna Maria Bello, Claudio Arra, Antonio Barbieri, Domenica Rea, Monica Fedele, Giuseppe De Palma, Paola Francesca, Gabriella De Vita, Laura Cerchia, Mario Monaco |
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| Přispěvatelé: | Vitiello, M., Palma, G., Monaco, M., Bello, A. M., Camorani, S., Francesca, P., Rea, D., Barbieri, A., Chiappetta, G., de Vita, G., Cerchia, L., Arra, C., Fedele, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
endocrine system lcsh:QH426-470 PATZ1 Tumor initiation Biology medicine.disease_cause Stem cell marker thyrospheres stem cell biology 03 medical and health sciences 0302 clinical medicine In vivo Genetics medicine thyroid cancer Thyroid cancer Genetics (clinical) Oncogene FRTL5 medicine.disease In vitro lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Stem cell Carcinogenesis Ras oncogene |
| Zdroj: | Genes (Basel) 10 (2019). doi:10.3390/genes10020127 info:cnr-pdr/source/autori:Vitiello, Michela; Palma, Giuseppe; Monaco, Mario; Bello, Anna Maria; Camorani, Simona; Francesca, Paola; Rea, Domenica; Barbieri, Antonio; Chiappetta, Gennaro; De Vita, Gabriella; Cerchia, Laura; Arra, Claudio; Fedele, Monica/titolo:Dual Oncogenic%2FAnti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-Ras(V12) Oncogene/doi:10.3390%2Fgenes10020127/rivista:Genes (Basel)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:10 Genes, Vol 10, Iss 2, p 127 (2019) Genes Volume 10 Issue 2 |
| Popis: | PATZ1 is a transcriptional factor downregulated in thyroid cancer whose re-expression in thyroid cancer cells leads to a partial reversion of the malignant phenotype, including the capacity to proliferate, migrate, and undergo epithelial-to-mesenchymal transition. We have recently shown that PATZ1 is specifically downregulated downstream of the Ras oncogenic signaling through miR-29b, and that restoration of PATZ1 in Ha-Ras transformed FRTL5 rat thyroid cells is able to inhibit their capacities to proliferate and migrate in vitro. Here, we analyzed the impact of PATZ1 expression on the in vivo tumorigenesis of these cells. Surprisingly, FRTL5-Ras-PATZ1 cells showed enhanced tumor initiation when engrafted in nude mice, even if their tumor growth rate was reduced compared to that of FRTL5-Ras control cells. To further investigate the cause of the enhanced tumor engraftment of FRTL5-Ras-PATZ1 cells, we analyzed the stem-like potential of these cells through their capacity to grow as thyrospheres. The results showed that restoration of PATZ1 expression in these cells increases stem cell markers&rsquo expression and self-renewal ability of the thyrospheres while limiting their growth capacity. Therefore, we suggest that PATZ1 may play a role in enhancing the stem cell potential of thyroid cancer cells, but, at the same time, it impairs the proliferation of non-stem cells. |
| Databáze: | OpenAIRE |
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