Simultaneous effect of organic modifier and physicochemical parameters of barbiturates on their retention on a narrow-bore PGC column
| Autor: | Ivan Mikšík, Esther Forgács, Zdenek Deyl, Tibor Cserháti, Adam Echardt |
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| Rok vydání: | 2004 |
| Předmět: |
Chemical Phenomena
Clinical Biochemistry chemistry.chemical_element Biochemistry Analytical Chemistry Dioxanes Structure-Activity Relationship chemistry.chemical_compound Phase (matter) Porosity Chromatography High Pressure Liquid Chromatography Barbituric acid Chemistry Physical Chemistry Cell Biology General Medicine Capacity factor Stationary phase Barbiturates Solvents Regression Analysis Graphite Theoretical plate Retention time Carbon |
| Zdroj: | Journal of Chromatography B. 800:259-262 |
| ISSN: | 1570-0232 |
| Popis: | The retention time of 22 barbituric acid derivatives was measured on a narrow-bore porous graphitized carbon (PGC) column using water-dioxane mixtures as mobile phases. The capacity factor (k), theoretical plate number (N), and asymmetry factor (AF) were calculated for each solute in each mobile phase. The relationships between chromatographic characteristics and physicochemical parameters of solutes were elucidated by stepwise regression analysis (SRA). SRA indicated that the binding of barbiturates to the PGC surface is of mixed character electrostatic and apolar interactive forces are equally involved. Sterical correspondence between the surface of the stationary phase and the solutes also exert a significant influence on the retention behavior. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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