Validation of a high-throughput and robust technique: BACs-on-beads assay (KaryoLite BoBs) for pre-implantation aneuploidy screening
| Autor: | Queenie Sum Yee Yeung, Qi Li, Wen Xu, Hong Li, Yvonne K. Kwok, Weiying Lu, Kwong Wai Choy, Cherry Kit Man Wong, Jian Ou, Grace Wing Shan Kong, Tin-Chiu Li, Yanlin Ma, Wei Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Validation study Aneuploidy Computational biology lcsh:Gynecology and obstetrics 03 medical and health sciences 0302 clinical medicine aCGH Medicine Humans Screening tool Genetic Testing Throughput (business) lcsh:RG1-991 Preimplantation Diagnosis Genetics Comparative Genomic Hybridization 030219 obstetrics & reproductive medicine business.industry Obstetrics and Gynecology BoB medicine.disease High-Throughput Screening Assays stomatognathic diseases 030104 developmental biology Strategic approach Karyotyping Karyolite PGS lipids (amino acids peptides and proteins) Detection rate business |
| Zdroj: | Taiwanese Journal of Obstetrics & Gynecology, Vol 56, Iss 4, Pp 514-520 (2017) |
| ISSN: | 1875-6263 |
| Popis: | Objective This study aims to validate the BACs-on-Beads (BoB) technology as a robust and high throughput method for pre-implantation genetic screening (PGS) for aneuploidy. Material and methods The performances with respect to the sensitivity, specificity, success rate and detection rate of this technique from new BoBs technology and traditional array chromosomal genomic hybridization (aCGH) were compared. And the use of BoBs as a screening tool for euploid embryos in PGS was evaluated. Result In the first part of validation study, there were total 75 embryos completed PGS by both BoBs and aCGH. The success rate of PGS was 97.4%, and the results showed 100% concordance between BoBs and aCGH for aneuploidy. In the second part, a total 219 embryos were involved. The success rate of PGS by BoBs was 100%. BoBs identified 28% (62/219) euploidy which were further confirmed to be euploidy by aCGH. Conclusion This new strategic approach using BoBs as a first tier PGS screening tool and aCGH as a confirmatory tool can increase the throughput of PGS with a reduced cost and time to meet the demand in high volume units. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|