MRSA screening by the Xpert MRSA PCR assay: pooling samples of the nose, throat, and groin increases the sensitivity of detection without increasing the laboratory costs

Autor: I. Nahimana, Giorgio Zanetti, Gilbert Greub, Dominique S. Blanc
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Microbiology (medical)
Methicillin-Resistant Staphylococcus aureus
medicine.medical_specialty
Carrier State/diagnosis
Polymerase Chain Reaction/methods
Pcr assay
Specimen Handling/methods
Nose
Groin
Methicillin-Resistant Staphylococcus aureus/isolation & purification
Polymerase Chain Reaction
Sensitivity and Specificity
Nose/microbiology
Specimen Handling
Mass Screening/methods
Internal medicine
Throat
medicine
Groin/microbiology
Polymerase Chain Reaction/economics
Specimen Handling/economics
Humans
Mass Screening
Molecular Diagnostic Techniques/economics
Bacteriological Techniques
Mass Screening/economics
Molecular Diagnostic Techniques/methods
business.industry
Staphylococcal Infections/diagnosis
Bacteriological Techniques/economics
General Medicine
Staphylococcal Infections
Surgery
Staphylococcal Infections/microbiology
Infectious Diseases
medicine.anatomical_structure
Molecular Diagnostic Techniques
Pharynx/microbiology
Carrier State
Bacteriological Techniques/methods
Costs and Cost Analysis
Carrier State/microbiology
Pharynx
Detection rate
business
Mrsa screening
Zdroj: European Journal of Clinical Microbiology and Infectious Diseases, vol. 32, no. 4, pp. 565-568
Popis: The performance of the Xpert MRSA polymerase chain reaction (PCR) assay on pooled nose, groin, and throat swabs (three nylon flocked eSwabs into one tube) was compared to culture by analyzing 5,546 samples. The sen- sitivity (0.78, 95 % confidence interval (CI) 0.73-0.82) and specificity (0.99, 95 % CI 0.98-0.99) were similar to the results from published studies on separated nose or other specimens. Thus, the performance of the Xpert MRSA assay was not affected by pooling the three specimens into one assay, allowing a higher detection rate without increasing laboratory costs, as compared to nose samples alone.
Databáze: OpenAIRE