Fabrication of microenvironments with different geometrical features for cell growth studies
| Autor: | Cleber Renato Mendonça, Enilza Maria Espreáfico, Laura Marise de Freitas, V. Tribuzi, Oriana I. Avila, Rodolfo Bortolozo Serafim, Valeria Valente, Adriano J. G. Otuka, Carla Raquel Fontana, M. H. Moraes |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Fabrication Tumor biology Cell growth Model system Nanotechnology Matrix (biology) Michigan Cancer Foundation-7 Green Fluorescent Protein Industrial and Manufacturing Engineering photopolimerization Cell density Biophysics Electrical and Electronic Engineering POLIMERIZAÇÃO Instrumentation Two-photon absortion microfabrication |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2015-03-18T15:56:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Studies of cell development in artificial microenvironments can contribute to understanding a series of physiological mechanisms that might be influenced by geometrical features of the microenvironment itself. In this work we applied two-photon polymerization to fabricate three-dimensional microenvironments composed of a matrix arrangement of microstructures (circular and square cross-sections pillars) with distinct spacing. Such microenvironments were used to evaluate the growth of Michigan Cancer Foundation-7 (MCF-7) cells that are commonly used as a model system to investigate fundamental aspects of the tumor biology. Our results reveal that the cell density decreases as the distance between structures in the environment is increased. Additionally, cell growth shows slightly better results for the microenvironments composed of circular cross-section structures. Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, BR-14801960 Araraquara, SP, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil NAP USP, CISBi Ctr Integrat Syst Biol, Sao Paulo, Brazil Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, BR-14801960 Araraquara, SP, Brazil FAPESP: 11/12399-0 FAPESP: 12/21807-7 |
| Databáze: | OpenAIRE |
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