Tissue expression of CD10 protein in colorectal carcinoma: correlation with the anatomopathological features of the tumor and with lymph node and liver metastases
| Autor: | Ricardo Artigiani Neto, Luís Cesar Fernandes, Monica Sznirer, Jaques Waisberg, George Joppert Netto, Levindo Alves de Oliveira, Flávio de Oliveira Lima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
CD10 antigens
Pathology medicine.medical_specialty medicine.drug_class Colorectal cancer neoplasm antigens RC799-869 metástase neoplásica Monoclonal antibody symbols.namesake neoplasm metastasis hemic and lymphatic diseases medicine Pathological Lymph node Fisher's exact test Tissue microarray business.industry Gastroenterology antígenos de neoplasia neoplasias colorretais Diseases of the digestive system. Gastroenterology colorectal neoplasms medicine.disease medicine.anatomical_structure prognóstico symbols Immunohistochemistry antígenos CD10 prognosis business Immunostaining |
| Zdroj: | Journal of Coloproctology (Rio de Janeiro) v.32 n.1 2012 Journal of Coloproctology (Rio de Janeiro. Online) Sociedade Brasileira de Coloproctologia (SBCP) instacron:SBCP Journal of Coloproctology, Vol 32, Iss 1, Pp 34-39 (2012) Journal of Coloproctology (Rio de Janeiro), Volume: 32, Issue: 1, Pages: 34-39, Published: MAR 2012 |
| Popis: | BACKGROUND: The reduced expression of CD10 may be related to unfavorable prognosis of patients with colorectal carcinoma. The authors analyzed the tissue immunostaining of CD10 protein in colorectal carcinoma and its relationship to clinicopathologic features. METHOD: In 130 patients submitted to colorectal carcinoma surgery, a tissue microarray block was obtained from the tumor and adjacent non-neoplastic mucosa and submitted to immunohistochemistry with monoclonal antibody CD10. The immunostaining was evaluated by semi-quantitative method, with stained cell count in percentage. The results were related to the location, anatomopathological features, presence of lymph node and hepatic metastases and TNM staging of the colorectal neoplasm. The statistical analysis was performed with the Mann-Whitney, Kruskal-Wallis and Fisher exact tests. RESULTS: The expression of CD10 marker was higher in colorectal tumor tissue than in adjacent non-neoplastic mucosa (pINTRODUÇÃO: A expressão reduzida de CD10 pode estar relacionada com prognóstico desfavorável de doentes com carcinoma colorretal. Analisou-se a imunoexpressão tecidual da proteína CD10 no carcinoma colorretal e sua relação com os aspectos clinicopatológicos. MÉTODO: Em 130 doentes operados por carcinoma colorretal, um bloco de tissue microarray foi obtido do tecido neoplásico e da mucosa não neoplásica adjacente e submetido ao estudo imuno-histoquímico com anticorpo monoclonal CD10. Avaliou-se a imunoexpressão por método semiquantitativo, com contagem do percentual de células coradas. Os resultados foram relacionados com a localização, aspectos anatomopatológicos, presença de metástases linfonodais e hepáticas e estadiamento TNM da neoplasia. O estudo estatístico foi realizado com os testes de Mann-Whitney, Kruskal-Wallis e exato de Fisher. RESULTADOS: A expressão do marcador CD10 foi maior no tecido do carcinoma colorretal do que na mucosa não neoplásica adjacente (p |
| Databáze: | OpenAIRE |
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