Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples
| Autor: | Mikael Lenz Strube, Mette Boye, Martin Weiss Nielsen, Tim Kåre Jensen, Kirstine Klitgaard, Worood D.H.M. Al-Medrasi, Anastasia Isbrand |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Virulence Factors 040301 veterinary sciences ved/biology.organism_classification_rank.species Cattle Diseases Porphyromonas medicine.disease_cause Microbiology 0403 veterinary science 03 medical and health sciences RNA Ribosomal 16S Next generation sequencing Fusobacterium necrophorum Gram-Negative Bacteria medicine Animals Bovine digital dermatitis Treponema 16S rRNA Skin General Veterinary biology medicine.diagnostic_test ved/biology Porphyromonas levii Microbiota RT-qPCR Digital dermatitis Biodiversity 04 agricultural and veterinary sciences General Medicine Mycoplasma biology.organism_classification medicine.disease veterinary(all) 030104 developmental biology Fusobacterium Cattle Digital Dermatitis Microbiome Gram-Negative Bacterial Infections Fluorescence in situ hybridization |
| Zdroj: | Weiss Nielsen, M, Strube, M L, Isbrand, A, Al-Medrasi, W D H M, Boye, M, Jensen, T K & Schou, K K 2016, ' Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples ', Veterinary Microbiology, vol. 186, pp. 139-149 . https://doi.org/10.1016/j.vetmic.2016.03.003 |
| DOI: | 10.1016/j.vetmic.2016.03.003 |
| Popis: | Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study the microbiomes of 27 DD-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed by fluorescence in situ hybridization (FISH), the results for Mycoplasma spp. were inconclusive. Extensive treponemal epidermal infiltration, constituting more than 90% of the total bacterial population, was observed in 24 of the 27 DD samples. F. necrophorum and P. levii were superficially located in the epidermal lesions and were present in only a subset of samples. RT-qPCR analysis showed that treponemes were also actively expressing a panel of virulence factors at the site of infection. Our results further support the hypothesis that species belonging to the genus Treponema are major pathogens of DD and also provide sufficient clues to motivate additional research into the role of M. fermentans, F. necrophorum and P. levii in the etiology of DD. |
| Databáze: | OpenAIRE |
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