Adaptive Roles of SSY1 and SIR3 During Cycles of Growth and Starvation in Saccharomyces cerevisiae Populations Enriched for Quiescent or Nonquiescent Cells
Autor: | Dominika Wloch-Salamon, Barbara Dunn, Dimitra Aggeli, Katarzyna Tomala |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Cell type Saccharomyces cerevisiae Proteins Population Saccharomyces cerevisiae Adaptation Biological Biology Investigations QH426-470 medicine.disease_cause Resting Phase Cell Cycle SPS pathway Evolution Molecular 03 medical and health sciences evolution medicine Genetics SSY1 quiescence Gene Silencing Amino Acids education Molecular Biology Gene Genetics (clinical) Silent Information Regulator Proteins Saccharomyces cerevisiae Mutation education.field_of_study Cell Cycle Intracellular Signaling Peptides and Proteins Membrane Proteins biology.organism_classification Phenotype Yeast Chromatin Endocytosis 030104 developmental biology SIR3 Gene-Environment Interaction Ploidy Metabolic Networks and Pathways Signal Transduction |
Zdroj: | G3: Genes, Genomes, Genetics, Vol 7, Iss 6, Pp 1899-1911 (2017) G3: Genes|Genomes|Genetics |
ISSN: | 2160-1836 |
Popis: | Over its evolutionary history, Saccharomyces cerevisiae has evolved to be well-adapted to fluctuating nutrient availability. In the presence of sufficient nutrients, yeast cells continue to proliferate, but upon starvation haploid yeast cells enter stationary phase and differentiate into nonquiescent (NQ) and quiescent (Q) cells. Q cells survive stress better than NQ cells and show greater viability when nutrient-rich conditions are restored. To investigate the genes that may be involved in the differentiation of Q and NQ cells, we serially propagated yeast populations that were enriched for either only Q or only NQ cell types over many repeated growth–starvation cycles. After 30 cycles (equivalent to 300 generations), each enriched population produced a higher proportion of the enriched cell type compared to the starting population, suggestive of adaptive change. We also observed differences in each population’s fitness suggesting possible tradeoffs: clones from NQ lines were better adapted to logarithmic growth, while clones from Q lines were better adapted to starvation. Whole-genome sequencing of clones from Q- and NQ-enriched lines revealed mutations in genes involved in the stress response and survival in limiting nutrients (ECM21, RSP5, MSN1, SIR4, and IRA2) in both Q and NQ lines, but also differences between the two lines: NQ line clones had recurrent independent mutations affecting the Ssy1p-Ptr3p-Ssy5p (SPS) amino acid sensing pathway, while Q line clones had recurrent, independent mutations in SIR3 and FAS1. Our results suggest that both sets of enriched-cell type lines responded to common, as well as distinct, selective pressures. |
Databáze: | OpenAIRE |
Externí odkaz: |