Effects of Glucagon, Neurotensin, and Vasoactive Intestinal Polypeptide on Somatostatin Release from Perifused Rat Hypothalamus*
| Autor: | Yuzuru Kato, Hiroo Imura, Hideki Katakami, Noboru Yanaihara, Norio Matsushita, Akira Shimatsu |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Vasoactive intestinal peptide Hypothalamus Neuropeptide Glucagon Gastrointestinal Hormones chemistry.chemical_compound Sincalide Endocrinology Internal medicine medicine Animals Neurotensin digestive oral and skin physiology Rats Inbred Strains Rats Somatostatin chemistry Sephadex Potassium Cholecystokinin hormones hormone substitutes and hormone antagonists Vasoactive Intestinal Peptide medicine.drug |
| Zdroj: | Endocrinology. 110:2113-2117 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | The effects of neuropeptides on the release of immunoreactive somatostatin (SRIF) from the rat hypothalamus were examined in vitro using a perifusion system. Twelve hypothalamic halves of male rats were placed on a Sephadex G-25 column and continuously eluted with Krebs-Ringer bicarbonate buffer, poH 7.4, at 37 C. A high potassium concentration (56 mM) stimulated SRIF release in a calcium-dependent manner. The infusion of glucagon (10(-7), 5 x 10(-7), and 10(-6) M) resulted in a dose-related increase in the release of SRIF. Neurotensin (10(-6) M) also stimulated SRIF release, whereas vasoactive intestinal polypeptide (10(-7) and 10(-6) M) inhibited SRIF release. SRIF release was not affected by cholecystokinin-octapeptide (10(-6) M), cholecystokinin-tetrapeptide (10(-6) M), or tRH (10(-6) M). These findings suggest that SRIF release from the rat hypothalamus is influenced by glucagon, neurotensin, and vasoactive intestinal polypeptide. |
| Databáze: | OpenAIRE |
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