Benign monoclonal expansion of CD8+ lymphocytes in HIV infection
| Autor: | Greta E Forster, Matthew Helbert, Tim Milne, Penelope R Smith, Denise Howe, Susanna J Wilkes, Jamie Cavenagh, Paul Sinnott |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Lymphocytosis Lymphocyte T cell Receptors Lymphocyte Homing HIV Infections Biology CD8-Positive T-Lymphocytes Pathology and Forensic Medicine T-Lymphocyte Subsets medicine Humans Immunity Cellular Diffuse infiltrative lymphocytosis syndrome T-cell receptor Antibodies Monoclonal General Medicine Gene rearrangement Syndrome Middle Aged medicine.disease Virology medicine.anatomical_structure Case-Control Studies Monoclonal Immunology Papers HIV-1 Female medicine.symptom CD8 |
| Zdroj: | Journal of clinical pathology. 53(3) |
| ISSN: | 0021-9746 |
| Popis: | Background —A transient expansion of the CD8+ T cell pool normally occurs in the early phase of HIV infection. Persistent expansion of this pool is observed in two related settings: diffuse infiltrative lymphocytosis syndrome (DILS) and HIV associated CD8+ lymphocytosis syndrome. Aim —To investigate a group of HIV infected patients with CD8+ lymphocytosis syndrome with particular emphasis on whether monoclonality was present. Methods —A group of 18 patients with HIV-1 infection and persistent circulating CD8+ lymphocytosis was compared with 21 HIV positive controls. Serum samples were tested for antinuclear antibodies, antibodies to extractable nuclear antigens, immunoglobulin levels, paraproteins, human T lymphotropic virus type 1 (HTLV-1), Epstein-Barr virus, and cytomegalovirus serology. Lymphocyte phenotyping and HLA-DR typing was performed, and T cell receptor (TCR) gene rearrangement studies used to identify monoclonal populations of T cells. CD4+ and CD8+ subsets of peripheral blood lymphocytes were purified to determine whether CD8+ populations inhibited HIV replication in autologous CD4+ cells. Results —A subgroup of patients with HIV-1 infection was found to have expanded populations of CD8+ T cell large granular lymphocytes persisting for 6 to 30 months. The consensus immunophenotype was CD4− CD8+ DR high CD11a+ CD11c+ CD16− CD28± CD56− CD57+, consistent with typical T cell large granular lymphocytes expressing cellular activation markers. Despite the finding of monoclonal TCR gene usage in five of 18 patients, there is evidence that the CD8+ expansions are reactive populations capable of mediating non-cytotoxic inhibition of HIV replication. Conclusions —A subgroup of HIV positive patients has CD8+ lymphocytosis, but despite the frequent occurrence of monoclonal TCR gene usage there is evidence that this represents an immune response to viral infection rather than a malignant disorder. |
| Databáze: | OpenAIRE |
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