EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS
| Autor: | V. I. Tynyanova, O. N. Podladchikova, V. A. Rykova, V. P. Zyuzina, E. P. Sokolova, G. V. Demidova, I. V. Morozova |
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| Jazyk: | ruština |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030213 general clinical medicine plasmids Lipopolysaccharide 030106 microbiology Cell Medicine (miscellaneous) Virulence Microbiology Cell wall 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Plasmid Extrachromosomal DNA medicine yersinia pestis biology lipopolysaccharide toxicity General Medicine biology.organism_classification QR1-502 medicine.anatomical_structure chemistry Yersinia pestis Bacteria |
| Zdroj: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 2, Pp 28-33 (2017) |
| ISSN: | 2686-7613 0372-9311 |
| Popis: | Aim. Elucidation of the role of extrachromosomal elements of heredity in manifestations of toxic properties of Yersinia pestis. Materials and methods. The study was carried out in vaccine strain Y. pestis EV76 (pMTl, pCDl, pPCPl) and non-plasmid variants of vaccine EV76 (pMTl\ pCDl', pPCPl') and virulent 231 (рМТГ, pCDl’, pPCPl') strains of Y. pestis. Presence of functionally active form of lipopolysaccharide (LPS) in the incubation medium of the bacteria was evaluated via toxicity of supernatant of Y. pestis for intact animals (infection-toxic shock) and mice sensitized by D-GalN. Results. 37°C cultures of Y. pestis EV76 containing a full amount of plasmids were established to release LPS into the environment. Non-plasmid variants of both vaccine and virulent strains of Y. pestis pMTl', pCD Г, рРСР 1 do not have this ability. Separation of LPS from cell wall was detected in live bacteria of plague infectious agent. This process is assumed to be coupled with translocation of proteins coded by pMTl, pCDl, pPCPl plasmids from the cell into the environment. Conclusion. Functional interconnection between extrachromosomal elements of heredity and toxic activity of Y. pestis LPS is established for the first time. |
| Databáze: | OpenAIRE |
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