Expression levels of A disintegrin and metalloproteases (ADAMs), and Th17-related cytokines and their association withHelicobacter pyloriinfection in patients with gastroduodenal diseases
| Autor: | Sanket Kumar Shukla, Jahanarah Khatoon, Uday C Ghoshal, Ravi Prakash Rai, Narendra Krishnani, Kashi N. Prasad |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Peptic Ulcer medicine.medical_specialty Biopsy Disintegrins medicine.medical_treatment Real-Time Polymerase Chain Reaction Gastroenterology Helicobacter Infections Pathogenesis 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Internal medicine Interleukin 23 Humans Immunology and Allergy Medicine Intestinal Mucosa Aged Aged 80 and over Helicobacter pylori General Immunology and Microbiology biology business.industry Interleukin General Medicine Middle Aged biology.organism_classification Interleukin 33 030104 developmental biology Infectious Diseases Real-time polymerase chain reaction Cytokine Gastric Mucosa 030220 oncology & carcinogenesis Metalloproteases Cytokines Female Interleukin 17 business |
| Zdroj: | Pathogens and Disease. 76 |
| ISSN: | 2049-632X |
| Popis: | Expression levels of A disintegrin and metalloproteases (ADAMs) (10 and 17) and Th17-related cytokines [interleukin (IL) 17A, IL-17F, IL-33, IL-23, IL-23R] were investigated by quantitative real time polymerase chain reaction in gastric biopsies of patients with different gastroduodenal pathologies in the presence and absence of Helicobacter pylori infection. Patients with gastric cancer (GC) (n = 70, intestinal-type 38 and diffuse type 32), peptic ulcer disease [n = 50, duodenal ulcer (DU) 16 and gastric ulcer (GU) 34] and functional dyspepsia (n = 120) were included in the study. Further, the expression levels of ADAMs and Th17 cytokines were correlated with H. pylori cytotoxin-associated genes pathogenicity island (cagPAI) status. Expression levels of ADAMs (10 and 17) and Th17-related cytokines (IL-17A, IL-23, IL-23R) were significantly higher in H. pylori-positive than in H. pylori-negative gastric biopsies. Significant increase in ADAM17 and Th17 cytokines (IL-17A and IL-23) expressions was observed in patients with GU and intestinal-type GC in the presence of H. pylori infection and in strains harbouring intact cagPAI. Expression levels of IL-17A, IL-23 and ADAM17 were strongly correlated with GU and intestinal-type GC and weakly with DU and diffuse-type GC in the presence of H. pylori infection. Higher expression levels of ADAM17 and Th17 cytokines (IL-17A and IL-23), and their strong correlation with GU and intestinal-type GC patients in the presence of H. pylori and its intact cagPAI status, suggest a possible role of strain specificity in the pathogenesis of these diseases. |
| Databáze: | OpenAIRE |
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