Oxygen-distribution within 3-D collagen I hydrogels for bone tissue engineering

Autor: Elizabeth R. Balmayor, Laura Heimann, Martin S. Gutbrod, Martijn van Griensven, Robert J. Meier, Gregor Liebsch, Paul Wolff
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
In situ
TENSION
MARROW HEMATOPOIETIC COMPARTMENT
HYPOXIA
In vitro oxygenation level
Oxygen
Bone tissue engineering
Tissue engineering
ADIPOSE
Tissue Engineering/methods
3-D gel construct
Cells
Cultured
Cultured
PROLIFERATION
Hydrogels
Apoptosis/physiology
APOPTOSIS
DIFFERENTIATION
Mechanics of Materials
Self-healing hydrogels
STEM-CELLS
Materials science
Oxygen/chemistry
Cells
chemistry.chemical_element
Cell Proliferation/physiology
GRADIENTS
MODELING PO(2) DISTRIBUTIONS
Bioengineering
Collagen Type I/chemistry
Collagen Type I
Biomaterials
03 medical and health sciences
In vivo
Animals
Humans
Cell Proliferation
Tissue Engineering
Mesenchymal Stem Cells/cytology
Mesenchymal stem cell
Hydrogels/chemistry
Collagen I
In vitro
030104 developmental biology
chemistry
Biophysics
Mesenchymal stem cells
Limiting oxygen concentration
Cattle
Zdroj: Materials Science & Engineering C-Materials for Biological Applications, 95, 422-427. Elsevier Science
ISSN: 0928-4931
DOI: 10.1016/j.msec.2018.02.015
Popis: Tissue engineering (TE) approaches typically envisage the structural and functional reconstitution of previously damaged tissue in situ. An adequate three-dimensional environment is therefore of fundamental importance for the designated cells associated to the scaffold material. The sufficient supply with nutrients and oxygen in vitro and in vivo mark thereby critical challenges of TE. In this study, we intended to analyse the level of locally dissolved oxygen within 3-D cell-loaded collagen I gels in vitro. For the analysis of the oxygen levels in situ, we employed an optical fibre-based micro sensor setup, as well as a camera supported non-invasive optical sensor foil based technique. These complementary analytical tools enable the identification, localization, and temporal follow-up investigation of specified regions of interest within TE constructs. Human adipose-derived mesenchymal stem cells (hAdMSCs) cultured in collagen I gels under normoxic conditions were analysed periodically and kinetically up to 70 days – thereby revealing dynamic changes of the level of dissolved oxygen inside the gel constructs. Dependent on the applied cell concentration, the in vitro oxygen concentration (cO2) within the gels reached physiological ranges (7–9%) after 21 days, or 35 days of culture. The minimal cO2 was measured after 35 days in vitro, featuring an oxygen level of 4.8 ± 1.3%. Upon prolonged culture, a plateau-like status of the cO2 around 8–9% established, indicating a change in the physiological activity of the cells under investigation. The expression patterns of BCL2, CASP3 and MCM5 revealed significant differences among the proliferative and apoptotic stages of the cell-loaded samples at the investigated time points of 7 and 70 days in culture. In summary, these data show the temporary dynamic nature of the oxygen distribution in cell-loaded gel constructs. The applied technique is an ideal tool for the evaluation of multiple parameters affecting the oxygen distribution in vitro. We conclude that it takes 5 weeks for establishing an equilibrium of cO2. Levels reached in a 3-D gel construct are comparable with physiological oxygenation ranges in bone-associated tissues.
Databáze: OpenAIRE
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